Linked Life Data

9099938

Source:http://linkedlifedata.com/resource/pubmed/id/9099938

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1997-5-5
pubmed:abstractText
Jak3 is a member of the Janus kinase family which plays an important role in cytokine signal transduction. Jak3 associates the gamma(c) chain of receptors for IL-2, IL-4, IL-7, IL-9 and IL-15, and is essential for the signal transduction of these cytokines. We have isolated Jak3 kinase from renal mesangial cells and demonstrated the constitutive expression of Jak3 in glomeruli in vivo. To investigate the physiological and pathological role of Jak3 in glomeruli, we prepared anti-Jak3 antibody and analysed the localization of Jak3 in glomeruli of renal biopsy samples from various nephritis patients and normal subjects. Among 61 nephritis patients and four normal subjects investigated in the present study, Jak3 was selectively localized to glomerular epithelia of IgA-N patients (14/34 cases) and focal glomerulosclerosis patients (1/5 cases), but not detected in minimal changes (n = 6), membranous glomerulonephropathy (n = 7), crescentic glomerulonephritis (n = 4), lupus nephritis patients (n = 5), and normal subjects (n = 4). The intense immunoreactivity for Jak3 is significantly associated with the decrease in creatinine clearance (81.5 +/- 10.4 ml/min versus 104.3 +/- 29.6 ml/min; P < 0.05, Student's t-test) and the increase in level of serum creatinine (1.13 +/- 0.33 mg/dl versus 0.75 +/- 0.23 mg/dl; P < 0.01, Student's t-test) in IgA-N patients. Furthermore, gamma(c) chain was concomitantly expressed with Jak3 in glomerular epithelia in vivo and in vitro, suggesting that signal transduction via gamma(c)-Jak3 cascade may be involved in the pathogenesis of glomerular injury of IgA-N. Taken together with the recent findings that IL-4-secreting T lymphocytes in affected glomeruli injure glomerular epithelium, the responsiveness of glomerular epithelium for IL-4 may be pathologically enhanced in IgA-N.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0009-9104
pubmed:author
pubmed:issnType
Print
pubmed:volume
104
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
517-24
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:9099938-Animals, pubmed-meshheading:9099938-Biopsy, pubmed-meshheading:9099938-Blotting, Northern, pubmed-meshheading:9099938-Cells, Cultured, pubmed-meshheading:9099938-Creatinine, pubmed-meshheading:9099938-Electrophoresis, Polyacrylamide Gel, pubmed-meshheading:9099938-Epithelium, pubmed-meshheading:9099938-Gene Expression Regulation, pubmed-meshheading:9099938-Glomerulonephritis, pubmed-meshheading:9099938-Glomerulonephritis, IGA, pubmed-meshheading:9099938-Glomerulonephritis, Membranous, pubmed-meshheading:9099938-Glomerulosclerosis, Focal Segmental, pubmed-meshheading:9099938-Humans, pubmed-meshheading:9099938-Immunohistochemistry, pubmed-meshheading:9099938-Janus Kinase 3, pubmed-meshheading:9099938-Kidney, pubmed-meshheading:9099938-Kidney Glomerulus, pubmed-meshheading:9099938-Lupus Nephritis, pubmed-meshheading:9099938-Polymerase Chain Reaction, pubmed-meshheading:9099938-Precipitin Tests, pubmed-meshheading:9099938-Protein Biosynthesis, pubmed-meshheading:9099938-Protein-Tyrosine Kinases, pubmed-meshheading:9099938-RNA, pubmed-meshheading:9099938-Rats, pubmed-meshheading:9099938-Recombinant Proteins, pubmed-meshheading:9099938-Recombination, Genetic, pubmed-meshheading:9099938-Sensitivity and Specificity, pubmed-meshheading:9099938-Signal Transduction
pubmed:year
1996
pubmed:articleTitle
Jak3 expression in glomerular epithelia of IgA nephropathy (IgA-N) patients.
pubmed:affiliation
Department of Molecular Pathology, Tokyo Metropolitan Institute of Gerontology, Itabashi-ku, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't