Linked Life Data

9089542

Source:http://linkedlifedata.com/resource/pubmed/id/9089542

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1997-9-29
pubmed:abstractText
The aims of this study were to determine if neural pathways between the duodenum and sphincter of Oddi are intramural, activated by duodenal electrical field stimulation (EFS) in vitro, and contain capsaicin-sensitive primary afferents. The possible involvement of cholinergic (muscarinic and/or nicotinic) and adrenergic receptors in these pathways were also investigated. Duodenal EFS (5-60 Hz, 70 V, 0.5 ms duration, 10 s train) at sites 2 cm oral and 2 cm anal to the sphincter of Oddi-duodenal junction produced frequency-dependent excitatory responses in the sphincter of Oddi, measured by manometry (n = 3). Excitatory responses from duodenal circular muscle were also evident. Tetrodotoxin (1 microM; n = 7) pretreatment abolished both sphincter of Oddi and duodenal responses to duodenal EFS. Crushing the duodenum between the site of stimulation and the sphincter of Oddi-duodenal junction also abolished sphincter of Oddi response. The sphincter of Oddi responses to duodenal EFS at the oral and anal sites were reduced by pretreatment with (i) atropine (100 nM: n = 7) to 19 +/- 6% (P < 0.05) and 22 +/- 8% (P < 0.05) of control respectively. (ii) hexamethonium (100 microM: n = 9) to 10 +/- 2% (P < 0.01) and 6.0 + 2.5% (P < 0.01) of control respectively and (iii) guanethidine (1 microM; n = 6) to 75 +/- 6% (P < 0.05) and 78 +/- 10% (P < 0.05) of control, respectively. Combined pretreatment with phentolamine and propranolol (both 1 microM; n = 7) was without effect, as was capsaicin (1 microM; n = 12) pretreatment. Excitatory intramural pathways between the sphincter of Oddi and the duodenum are primarily cholinergic in nature and contain an adrenergic component. Capsaicin-sensitive primary afferents are not involved.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0165-1838
pubmed:author
pubmed:issnType
Print
pubmed:day
19
pubmed:volume
63
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
77-84
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:9089542-Adrenergic Agents, pubmed-meshheading:9089542-Animals, pubmed-meshheading:9089542-Capsaicin, pubmed-meshheading:9089542-Duodenum, pubmed-meshheading:9089542-Electric Stimulation, pubmed-meshheading:9089542-Female, pubmed-meshheading:9089542-Ganglionic Blockers, pubmed-meshheading:9089542-Guanethidine, pubmed-meshheading:9089542-Hexamethonium, pubmed-meshheading:9089542-Male, pubmed-meshheading:9089542-Marsupialia, pubmed-meshheading:9089542-Muscle Contraction, pubmed-meshheading:9089542-Neural Pathways, pubmed-meshheading:9089542-Neurotoxins, pubmed-meshheading:9089542-Phentolamine, pubmed-meshheading:9089542-Propranolol, pubmed-meshheading:9089542-Species Specificity, pubmed-meshheading:9089542-Sphincter of Oddi, pubmed-meshheading:9089542-Sympatholytics, pubmed-meshheading:9089542-Tetrodotoxin
pubmed:year
1997
pubmed:articleTitle
In vitro characterisation of intramural neural pathways between the duodenum and the sphincter of Oddi of the brush-tailed possum.
pubmed:affiliation
Department of Surgery, Flinders Medical Centre, Bedford Park, Australia.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't