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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1997-10-16
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pubmed:abstractText |
HLA-A2 heavy chain and beta 2-microglobulin were expressed in Escherichia coli, and refolded in the presence of peptides derived from HIV-1 RT and gag proteins. When recombinant HLA-A2 molecules were attached to cells lacking HLA-A2, the cells became susceptible to lysis by HLA-A2-restricted cytotoxic T lymphocyte (CTL) clones specific for peptides derived from RT and gag proteins. Limiting dilution analyses of peripheral blood mononuclear cells from HIV-1-infected individuals showed that the recombinant HLA-A2 peptide complexes covalently immobilized on microspheres stimulated the development of HLA-A2 peptide-specific CTL. Preformed HLA-peptide complexes may provide an alternative to immunization procedures that depend upon intracellular processing of antigen to elicit T cell responses.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0953-8178
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
9
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
451-9
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:9088983-Antigen Presentation,
pubmed-meshheading:9088983-Gene Products, gag,
pubmed-meshheading:9088983-HIV Antigens,
pubmed-meshheading:9088983-HIV Reverse Transcriptase,
pubmed-meshheading:9088983-HIV-1,
pubmed-meshheading:9088983-HLA-A2 Antigen,
pubmed-meshheading:9088983-Humans,
pubmed-meshheading:9088983-Lymphocyte Activation,
pubmed-meshheading:9088983-Microspheres,
pubmed-meshheading:9088983-Peptide Fragments,
pubmed-meshheading:9088983-Recombinant Fusion Proteins,
pubmed-meshheading:9088983-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:9088983-beta 2-Microglobulin
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pubmed:year |
1997
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pubmed:articleTitle |
Stimulation of human cytotoxic T cells with HIV-1-derived peptides presented by recombinant HLA-A2 peptide complexes.
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pubmed:affiliation |
Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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