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rdf:type |
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lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0017626,
umls-concept:C0027836,
umls-concept:C0037083,
umls-concept:C0040649,
umls-concept:C0185117,
umls-concept:C0205263,
umls-concept:C0205548,
umls-concept:C0207071,
umls-concept:C1150611,
umls-concept:C1515877,
umls-concept:C1707310,
umls-concept:C1879547,
umls-concept:C2911684
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pubmed:issue |
4
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pubmed:dateCreated |
1997-4-28
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pubmed:abstractText |
In recent reports, ciliary neurotrophic factor (CNTF) has been implicated as an injury factor involved in regulating astrogliosis in the CNS. In this study, we used a rat oligodendroglial progenitor cell line that is highly responsive to CNTF to examine CNTF-induced alterations that may play a role in activation of the glial fibrillary acidic protein (GFAP) gene. We determined that CNTF induces the transient translocation of Stat1 alpha/p91 to the nucleus. This nuclear translocation was followed by GFAP promoter activation and an up-regulation of GFAP mRNA and protein. Level of CNTF-alpha receptor mRNA, however, were unaffected by addition of the ligand. Transfection studies using an upstream 5'-flanking, 1.9-kb rat GFAP promoter linked to a luciferase reporter gene revealed CNTF-induced transcriptional activation within 1 h of ligand exposure. Moreover, serial-deleted constructs identified a distal (-1,857 to -1,546 bp) and a proximal (-384 to -106 bp) region as being important for CNTF-induced GFAP promoter activation. These two regions showed a strong degree of overlap for CNTF- and serum-induced activation of the GFAP gene. Analysis of the two regions revealed several cis-elements that are thought to be involved in GFAP regulation and/or the regulation of other genes by members of the interleukin-6 family of cytokines. Moreover, we are the first to report the presence of several putative CNTF-responsive elements within our identified distal and proximal regions in the GFAP gene promoter.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acute-Phase Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Blood Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Ciliary Neurotrophic Factor,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Glial Fibrillary Acidic Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Jak1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Janus Kinase 1,
http://linkedlifedata.com/resource/pubmed/chemical/Luciferases,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Growth Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/STAT3 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Stat3 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0022-3042
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
68
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1413-23
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:9084411-Acute-Phase Proteins,
pubmed-meshheading:9084411-Animals,
pubmed-meshheading:9084411-Blood Proteins,
pubmed-meshheading:9084411-Blotting, Northern,
pubmed-meshheading:9084411-Cell Line,
pubmed-meshheading:9084411-Ciliary Neurotrophic Factor,
pubmed-meshheading:9084411-DNA-Binding Proteins,
pubmed-meshheading:9084411-Gene Deletion,
pubmed-meshheading:9084411-Gene Expression Regulation,
pubmed-meshheading:9084411-Genes, Reporter,
pubmed-meshheading:9084411-Glial Fibrillary Acidic Protein,
pubmed-meshheading:9084411-Janus Kinase 1,
pubmed-meshheading:9084411-Luciferases,
pubmed-meshheading:9084411-Mutagenesis,
pubmed-meshheading:9084411-Nerve Growth Factors,
pubmed-meshheading:9084411-Nerve Tissue Proteins,
pubmed-meshheading:9084411-Oligodendroglia,
pubmed-meshheading:9084411-Promoter Regions, Genetic,
pubmed-meshheading:9084411-Protein-Tyrosine Kinases,
pubmed-meshheading:9084411-RNA, Messenger,
pubmed-meshheading:9084411-Rats,
pubmed-meshheading:9084411-STAT3 Transcription Factor,
pubmed-meshheading:9084411-Signal Transduction,
pubmed-meshheading:9084411-Stem Cells,
pubmed-meshheading:9084411-Trans-Activators,
pubmed-meshheading:9084411-Transcription, Genetic,
pubmed-meshheading:9084411-Transfection
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pubmed:year |
1997
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pubmed:articleTitle |
Ciliary neurotrophic factor activates JAK/Stat signal transduction cascade and induces transcriptional expression of glial fibrillary acidic protein in glial cells.
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pubmed:affiliation |
Department of Neurobiology, UCLA School of Medicine 90024-1764, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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