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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:dateCreated |
1997-5-29
|
| pubmed:databankReference |
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X87177,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X87178,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X87179,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X87180,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X87181,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X87182,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X87183,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X87184,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X87185,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X87186,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X87187,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X87188,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X87189,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X87190,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X87191
|
| pubmed:abstractText |
The 16S-23S and 23S-5S rRNA spacer DNA regions (spacer regions 1 and 2, respectively) from Enterococcus faecalis, Enterococcus faecium, Enterococcus hirae, Enterococcus durans and Enterococcus mundtii were amplified by PCR. Their nucleotide sequences were established and a secondary structure model showing the interaction between the two spacer regions was built. Whereas lactococci and Streptococcus sensu stricto are characterized by a single type of spacer region 1, the enterococci show a high degree of variability in this region; thus the spacer regions 1 with and without tRNA(Ala) were characterized. However, as shown for lactococci and Streptococcus sensu stricto, the tRNA(Ala) gene does not encode the 3'-terminal CCA trinucleotide. A putative antitermination signal is found downstream from the tRNA(Ala) gene. Based on comparison with Lactococcus lactis and Streptococcus thermophilus, a double-stranded processing stem is proposed. In E, hirae, one of the three different types of spacer region 1 contains no tRNA(Ala), but displays a 107 nt insertion that forms a long stem-loop structure. A similar insertion (115 nt in length) was found in E. faecium and base compensatory mutations preserve the ability to form the long stem-loop structure. Such insertions may correspond to mobile intervening sequences, as found in the 23S rRNA coding sequences of some Gram-negative bacteria. The spacer regions 1 and 2 from the three subgroups of streptococci were compared, and except for the tRNA(Ala) gene and the double-stranded processing sites, little similarity was found, which opens large possibilities for future development of DNA-based typing methods.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
1350-0872
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
143 ( Pt 3)
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
823-34
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9084166-Base Sequence,
pubmed-meshheading:9084166-Enterococcus,
pubmed-meshheading:9084166-Molecular Sequence Data,
pubmed-meshheading:9084166-Nucleic Acid Conformation,
pubmed-meshheading:9084166-RNA, Bacterial,
pubmed-meshheading:9084166-RNA, Ribosomal,
pubmed-meshheading:9084166-RNA, Transfer,
pubmed-meshheading:9084166-Sequence Alignment
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Primary and secondary structures of rRNA spacer regions in enterococci.
|
| pubmed:affiliation |
Laboratoire d'Enzymologie et de Génie Génétique, URA CNRS 457, Université de Nancy I, Vandceuvre-Lès-Nancy, France.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|