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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1997-5-12
|
| pubmed:abstractText |
Two Chinese hamster ovary cell (CHO-K1) mutants selected for defective glutamate transport via system X-AG are also highly permeable to small neutral molecules. Light microscopy demonstrated that exposure of one of these mutants, Ed-A1, to hypo-osmotic medium led to extremely rapid swelling, presumably due to increased water flux. When placed in 20% saline, Ed-A1 cells swelled to three times their original volume within 15 sec, a sixfold larger increase than parental CHO-K1. In spite of this rapid volume increase, mutant and wild-type cells remained viable for 20 min in dilute saline. A regulatory volume decrease in Ed-A1, and the continual swelling of CHO-K1, resulted in the two cells achieving equal size after 5 min in 20% saline. The time course of these volume changes permitted analysis of large numbers of cells by a hydrodynamic technique, steric field flow fractionation (FFF). Steric FFF demonstrated the expected inhibition of osmotic swelling of human erythrocytes by the mercurial, p-chloromercuribenzenesulfonic acid (PCMBS). However, PCMBS increased the apparent swelling rate of Ed-A1 and CHO-K1, suggesting that an aquaporin-like molecule is not responsible for any significant fraction of the water fluxes into either line. PCMBS also strongly inhibited aspartate transport by system X-AG. By taking advantage of their different swelling rates in hypotonic medium, steric FFF can separate mixtures of CHO-K1 and Ed-A1.
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| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/4-Chloromercuribenzenesulfonate,
http://linkedlifedata.com/resource/pubmed/chemical/Amino Acid Transport System X-AG,
http://linkedlifedata.com/resource/pubmed/chemical/Aspartic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Glutamate Plasma Membrane...,
http://linkedlifedata.com/resource/pubmed/chemical/Glutamic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Hypotonic Solutions,
http://linkedlifedata.com/resource/pubmed/chemical/Symporters
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0022-2631
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
156
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
131-9
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:9075644-4-Chloromercuribenzenesulfonate,
pubmed-meshheading:9075644-Adult,
pubmed-meshheading:9075644-Amino Acid Transport System X-AG,
pubmed-meshheading:9075644-Animals,
pubmed-meshheading:9075644-Aspartic Acid,
pubmed-meshheading:9075644-Biological Transport,
pubmed-meshheading:9075644-CHO Cells,
pubmed-meshheading:9075644-Carrier Proteins,
pubmed-meshheading:9075644-Cell Size,
pubmed-meshheading:9075644-Cricetinae,
pubmed-meshheading:9075644-Cricetulus,
pubmed-meshheading:9075644-Erythrocytes,
pubmed-meshheading:9075644-Glutamate Plasma Membrane Transport Proteins,
pubmed-meshheading:9075644-Glutamic Acid,
pubmed-meshheading:9075644-Humans,
pubmed-meshheading:9075644-Hypotonic Solutions,
pubmed-meshheading:9075644-Osmotic Fragility,
pubmed-meshheading:9075644-Osmotic Pressure,
pubmed-meshheading:9075644-Symporters
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Rapid swelling of a CHO-K1 aspartate/glutamate transport mutant in hypo-osmotic medium.
|
| pubmed:affiliation |
Department of Bioengineering, University of Utah, Salt Lake City, UT 84112, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
|