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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1997-4-15
|
| pubmed:abstractText |
A growth-inhibitory substance found in the culture of a B-precursor leukemia cell line, NALM-20, was purified from the serum-free culture medium and identified as arginine deiminase derived from Mycoplasma arginini (EC 3.5.3.6). Arginine deiminase strongly inhibited, in a dose-dependent manner, the growth of human T cells and T lymphoblastoid cell lines, but not that of B-precursor and myeloid cell lines. The addition of L-arginine completely restored the growth of T lymphoblastoid cells that had been inhibited by arginine deiminase. The addition of L-ornithine also partially restored it. This enzyme suppressed interleukin-2 (IL-2) production and IL-2 receptor expression in T cells stimulated by non-specific mitogens. The morphologic features of dying cells and DNA fragmentation indicated that arginine deiminase induced apoptotic cell death in T lymphoblasts. Cell cycle analysis revealed that G1-->S transition was blocked in cell treated with arginine deiminase, accompanied by the increase of apoptotic nuclei in the sub-G1 fraction. In conclusion, the deletion of the essential nutrient L-arginine by arginine deiminase significantly inhibited cell growth and activation in T lymphoblasts, accompanied by the induction of apoptotic cell death.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0925-5710
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
65
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
129-41
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9071817-Apoptosis,
pubmed-meshheading:9071817-Cell Cycle,
pubmed-meshheading:9071817-Cells, Cultured,
pubmed-meshheading:9071817-Dose-Response Relationship, Drug,
pubmed-meshheading:9071817-Humans,
pubmed-meshheading:9071817-Hydrolases,
pubmed-meshheading:9071817-Mycoplasma,
pubmed-meshheading:9071817-T-Lymphocytes
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Apoptotic cell death of human T lymphoblastoid cells induced by arginine deiminase.
|
| pubmed:affiliation |
Department of Pediatrics, Mie University School of Medicine, Japan.
|
| pubmed:publicationType |
Journal Article
|