Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1997-3-31
pubmed:databankReference
pubmed:abstractText
The hairpin structure at the 3' end of animal histone mRNAs controls histone RNA 3' processing, nucleocytoplasmic transport, translation and stability of histone mRNA. Functionally overlapping, if not identical, proteins binding to the histone RNA hairpin have been identified in nuclear and polysomal extracts. Our own results indicated that these hairpin binding proteins (HBPs) bind their target RNA as monomers and that the resulting ribonucleoprotein complexes are extremely stable. These features prompted us to select for HBP-encoding human cDNAs by RNA-mediated three-hybrid selection in Saccharomyces cerevesiae. Whole cell extract from one selected clone contained a Gal4 fusion protein that interacted with histone hairpin RNA in a sequence- and structure-specific manner similar to a fraction enriched for bovine HBP, indicating that the cDNA encoded HBP. DNA sequence analysis revealed that the coding sequence did not contain any known RNA binding motifs. The HBP gene is composed of eight exons covering 19.5 kb on the short arm of chromosome 4. Translation of the HBP open reading frame in vitro produced a 43 kDa protein with RNA binding specificity identical to murine or bovine HBP. In addition, recombinant HBP expressed in S. cerevisiae was functional in histone pre-mRNA processing, confirming that we have indeed identified the human HBP gene.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9049306-1338771, http://linkedlifedata.com/resource/pubmed/commentcorrection/9049306-1461736, http://linkedlifedata.com/resource/pubmed/commentcorrection/9049306-1531633, http://linkedlifedata.com/resource/pubmed/commentcorrection/9049306-1561104, http://linkedlifedata.com/resource/pubmed/commentcorrection/9049306-1633440, http://linkedlifedata.com/resource/pubmed/commentcorrection/9049306-1833188, http://linkedlifedata.com/resource/pubmed/commentcorrection/9049306-1834994, http://linkedlifedata.com/resource/pubmed/commentcorrection/9049306-1856231, http://linkedlifedata.com/resource/pubmed/commentcorrection/9049306-1883210, http://linkedlifedata.com/resource/pubmed/commentcorrection/9049306-1885007, http://linkedlifedata.com/resource/pubmed/commentcorrection/9049306-2005802, http://linkedlifedata.com/resource/pubmed/commentcorrection/9049306-2166220, http://linkedlifedata.com/resource/pubmed/commentcorrection/9049306-2231712, http://linkedlifedata.com/resource/pubmed/commentcorrection/9049306-2547163, http://linkedlifedata.com/resource/pubmed/commentcorrection/9049306-271968, http://linkedlifedata.com/resource/pubmed/commentcorrection/9049306-2734288, http://linkedlifedata.com/resource/pubmed/commentcorrection/9049306-2779556, http://linkedlifedata.com/resource/pubmed/commentcorrection/9049306-2955216, http://linkedlifedata.com/resource/pubmed/commentcorrection/9049306-3022153, http://linkedlifedata.com/resource/pubmed/commentcorrection/9049306-3070846, http://linkedlifedata.com/resource/pubmed/commentcorrection/9049306-3070866, http://linkedlifedata.com/resource/pubmed/commentcorrection/9049306-3319781, http://linkedlifedata.com/resource/pubmed/commentcorrection/9049306-3437896, http://linkedlifedata.com/resource/pubmed/commentcorrection/9049306-3684574, http://linkedlifedata.com/resource/pubmed/commentcorrection/9049306-3943125, http://linkedlifedata.com/resource/pubmed/commentcorrection/9049306-6194891, http://linkedlifedata.com/resource/pubmed/commentcorrection/9049306-7543408, http://linkedlifedata.com/resource/pubmed/commentcorrection/9049306-7828876, http://linkedlifedata.com/resource/pubmed/commentcorrection/9049306-7899087, http://linkedlifedata.com/resource/pubmed/commentcorrection/9049306-7937126, http://linkedlifedata.com/resource/pubmed/commentcorrection/9049306-8114706, http://linkedlifedata.com/resource/pubmed/commentcorrection/9049306-8384581, http://linkedlifedata.com/resource/pubmed/commentcorrection/9049306-8479907, http://linkedlifedata.com/resource/pubmed/commentcorrection/9049306-8548656, http://linkedlifedata.com/resource/pubmed/commentcorrection/9049306-8652556, http://linkedlifedata.com/resource/pubmed/commentcorrection/9049306-8710898, http://linkedlifedata.com/resource/pubmed/commentcorrection/9049306-8957003
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0261-4189
pubmed:author
pubmed:issnType
Print
pubmed:day
17
pubmed:volume
16
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
769-78
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:9049306-Humans, pubmed-meshheading:9049306-Animals, pubmed-meshheading:9049306-Cattle, pubmed-meshheading:9049306-Histones, pubmed-meshheading:9049306-Saccharomyces cerevisiae, pubmed-meshheading:9049306-Genes, pubmed-meshheading:9049306-RNA, pubmed-meshheading:9049306-Base Sequence, pubmed-meshheading:9049306-RNA, Messenger, pubmed-meshheading:9049306-Amino Acid Sequence, pubmed-meshheading:9049306-Chromosome Mapping, pubmed-meshheading:9049306-Organ Specificity, pubmed-meshheading:9049306-Genetic Complementation Test, pubmed-meshheading:9049306-Molecular Sequence Data, pubmed-meshheading:9049306-Nuclear Proteins, pubmed-meshheading:9049306-Nucleic Acid Conformation, pubmed-meshheading:9049306-Chromosomes, Human, Pair 4, pubmed-meshheading:9049306-Cloning, Molecular, pubmed-meshheading:9049306-Sequence Analysis, DNA
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