Linked Life Data

9042345

Source:http://linkedlifedata.com/resource/pubmed/id/9042345

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1997-3-20
pubmed:abstractText
Fluorescent styrylpyridinium dyes have recently been suggested as probes of the membrane dipole potential and of the kinetics of electrogenic ion pumps. It is necessary, however, to be able to confidently attribute observed fluorescence changes to electrical effects alone and avoid interference from changes in membrane fluidity. Furthermore, the effect of the dyes themselves on the dipole potential must be investigated. The effect of membrane fluidity on the fluorescence excitation and emission spectra of the dyes RH421 and di-8-ANEPPS have been investigated in lipid vesicles by temperature scans between 15 and 60 degrees C. Both dyes show significant temperature-dependent shifts of their excitation spectra, the magnitude of which depend on the emission wavelength and on the lipid structure. In order to eliminate membrane fluidity effects, fluorescence must be detected at the red edge of the emission spectrum; in this case 670 nm. In order to avoid dye-induced shifts of the excitation spectra of membrane-bound dye, an excess molar ratio of lipid to dye of at least 200-fold is necessary. Fluorescence ratio measurements indicate qualitatively that dimyristoylphosphatidylcholine has a significantly higher dipole potential than that of dioleoylphosphatidylcholine.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
31
pubmed:volume
1323
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
223-39
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Optical detection of membrane dipole potential: avoidance of fluidity and dye-induced effects.
pubmed:affiliation
Department of Biophysical Chemistry, Max-Planck-Institut für Biophysik, Frankfurt am Main, Germany. clarker@kennedy.mpibp.uni-frankfurt.de
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't