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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1997-3-13
|
| pubmed:abstractText |
We investigated the injurious effects of reactive oxygen metabolites on the intestinal epithelium and the possible protective role played by two olive oil phenolic compounds, (3,4-dihydroxyphenyl)ethanol and (p-hydroxyphenyl)ethanol, using the Caco-2 human cell line. We induced oxidative stress in the apical compartment, either by the addition of 10 mmol/L H2O2 or by the action of 10 U/L xanthine oxidase in the presence of xanthine (250 micromol/L); after the incubation, we evaluated the cellular and molecular alterations. Both treatments produced significant decreases in Caco-2 viability as assessed by the neutral red assay. Furthermore, we observed a significant increase in malondialdehyde intracellular concentration and paracellular inulin transport, indicating the occurrence of lipid peroxidation and monolayer permeability changes, respectively. The H2O2-induced alterations were completely prevented by preincubating Caco-2 cells with (3,4-dihydroxyphenyl)ethanol (250 micromol/L); when the oxidative stress was induced by xanthine oxidase, complete protection was obtained at a concentration of polyphenol as small as 100 micromol/L. In contrast, (p-hydroxyphenyl)ethanol was ineffective up to a concentration of 500 micromol/L. Our data demonstrate that (3,4-dihydroxyphenyl)ethanol can act as a biological antioxidant in a cell culture experimental model and that the ortho-dihydroxy moiety of the molecule is essential for antioxidant activity. This study suggests that dietary intake of olive oil polyphenols may lower the risk of reactive oxygen metabolite-mediated diseases such as some gastrointestinal diseases and atherosclerosis.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/3,4-dihydroxyphenylethanol,
http://linkedlifedata.com/resource/pubmed/chemical/4-hydroxyphenylethanol,
http://linkedlifedata.com/resource/pubmed/chemical/Antioxidants,
http://linkedlifedata.com/resource/pubmed/chemical/Dietary Fats, Unsaturated,
http://linkedlifedata.com/resource/pubmed/chemical/Hydrogen Peroxide,
http://linkedlifedata.com/resource/pubmed/chemical/Inulin,
http://linkedlifedata.com/resource/pubmed/chemical/Malondialdehyde,
http://linkedlifedata.com/resource/pubmed/chemical/Oxidants,
http://linkedlifedata.com/resource/pubmed/chemical/Phenylethyl Alcohol,
http://linkedlifedata.com/resource/pubmed/chemical/Plant Oils,
http://linkedlifedata.com/resource/pubmed/chemical/Reactive Oxygen Species,
http://linkedlifedata.com/resource/pubmed/chemical/Xanthine,
http://linkedlifedata.com/resource/pubmed/chemical/Xanthine Oxidase,
http://linkedlifedata.com/resource/pubmed/chemical/Xanthines,
http://linkedlifedata.com/resource/pubmed/chemical/olive oil
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0022-3166
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
127
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
286-92
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9039829-Antioxidants,
pubmed-meshheading:9039829-Caco-2 Cells,
pubmed-meshheading:9039829-Cell Survival,
pubmed-meshheading:9039829-Dietary Fats, Unsaturated,
pubmed-meshheading:9039829-Humans,
pubmed-meshheading:9039829-Hydrogen Peroxide,
pubmed-meshheading:9039829-Intestines,
pubmed-meshheading:9039829-Inulin,
pubmed-meshheading:9039829-Lipid Peroxidation,
pubmed-meshheading:9039829-Malondialdehyde,
pubmed-meshheading:9039829-Oxidants,
pubmed-meshheading:9039829-Oxidative Stress,
pubmed-meshheading:9039829-Phenylethyl Alcohol,
pubmed-meshheading:9039829-Plant Oils,
pubmed-meshheading:9039829-Reactive Oxygen Species,
pubmed-meshheading:9039829-Xanthine,
pubmed-meshheading:9039829-Xanthine Oxidase,
pubmed-meshheading:9039829-Xanthines
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
The protective effect of the olive oil polyphenol (3,4-dihydroxyphenyl)-ethanol counteracts reactive oxygen metabolite-induced cytotoxicity in Caco-2 cells.
|
| pubmed:affiliation |
Institute of Biochemistry of Macromolecules, Medical School, Second University of Naples, Italy.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|