Linked Life Data

9037182

Source:http://linkedlifedata.com/resource/pubmed/id/9037182

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2-3
pubmed:dateCreated
1997-3-20
pubmed:abstractText
Site-directed mutants of yeast ATPase were studied after introduction of mutant alleles into a yeast strain where these alleles were constitutively expressed and the expression of the wild-type chromosomal ATPase gene was turned off. One objection to this constitutive expression system was made apparent recently, as dominant lethal mutations are lost by gene conversion with the wild-type allele during the process. Here, the phenotypes of the mutant alleles, which were studied in a constitutive expression system, are re-evaluated under conditions in which these site-directed mutants are conditionally expressed. We show that 12 of 25 site-directed mutations previously described are actually dominant lethal alleles. In addition, we show that dominant mutant proteins interfere with transport of wild-type ATPase to the plasma membrane.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0014-5793
pubmed:author
pubmed:issnType
Print
pubmed:day
3
pubmed:volume
402
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
136-40
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Characterization of dominant lethal mutations in the yeast plasma membrane H+-ATPase gene.
pubmed:affiliation
Departamento de Bioquímica de la Facultad de Medicina de la Universidad Autónoma de Madrid, Spain. FPortillo@biomed.iib.uam.es
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't