Linked Life Data

9033279

Source:http://linkedlifedata.com/resource/pubmed/id/9033279

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1997-3-18
pubmed:abstractText
Several recent studies have reported detection of HTLV-I genetic sequences in patients with cutaneous T-cell lymphoma (CTCL) including mycosis fungoides and Sezary syndrome. The purpose of this study was to determine whether HTLV-I was detectable in lesional tissues of patients suffering from diseases known to be associated with CTCL. Thirty-five cases were obtained from diverse geographical locations including Ohio, California, Switzerland, and Japan. Six of them had concurrent CTCL. Cases were analyzed using a combination of genomic polymerase chain reaction (PCR)/ Southern blot, dot blot, and Southern blot analyses. All assays were specific for HTLV-I provirus. Sensitivity ranged from approximately 10(-6) for PCR-based studies to 10(-2) for unamplified genomic blotting. Lesional DNA from patients with lymphomatoid papulosis (fourteen cases), Hodgkin's disease (twelve cases), and CD30+ large-cell lymphoma (nine cases) was tested for the HTLV-I proviral pX region using a genomic PCR assay followed by confirmatory Southern blot analysis with a nested oligonucleotide pX probe. All cases were uniformly negative. All of the Hodgkin's disease cases, eight of the large-cell lymphoma cases, and six of the lymphomatoid papulosis cases were then subjected to dot blot analysis of genomic DNA using a full-length HTLV-I proviral DNA probe that spans all regions of the HTLV-I genome. Again, all cases were negative. Finally, eleven of the Hodgkin's disease cases were also subjected to Southern blot analysis of EcoRI-digested genomic DNA using the same full-length HTLV-I probe. Once again, all cases were negative. These findings indicated that, despite utilization of a variety of sensitive and specific molecular biological methods, HTLV-I genetic sequences were not detectable in patients with CTCL-associated lymphoproliferative disorders. These results strongly suggest that the HTLV-I retrovirus is not involved in the pathogenesis of these diseases.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9033279-1353704, http://linkedlifedata.com/resource/pubmed/commentcorrection/9033279-1532439, http://linkedlifedata.com/resource/pubmed/commentcorrection/9033279-1645581, http://linkedlifedata.com/resource/pubmed/commentcorrection/9033279-1655230, http://linkedlifedata.com/resource/pubmed/commentcorrection/9033279-1763059, http://linkedlifedata.com/resource/pubmed/commentcorrection/9033279-17801282, http://linkedlifedata.com/resource/pubmed/commentcorrection/9033279-1857968, http://linkedlifedata.com/resource/pubmed/commentcorrection/9033279-1881904, http://linkedlifedata.com/resource/pubmed/commentcorrection/9033279-2004007, http://linkedlifedata.com/resource/pubmed/commentcorrection/9033279-2175544, http://linkedlifedata.com/resource/pubmed/commentcorrection/9033279-2825353, http://linkedlifedata.com/resource/pubmed/commentcorrection/9033279-7615976, http://linkedlifedata.com/resource/pubmed/commentcorrection/9033279-7774890, http://linkedlifedata.com/resource/pubmed/commentcorrection/9033279-7860737, http://linkedlifedata.com/resource/pubmed/commentcorrection/9033279-8396607, http://linkedlifedata.com/resource/pubmed/commentcorrection/9033279-8451645, http://linkedlifedata.com/resource/pubmed/commentcorrection/9033279-8781433
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0002-9440
pubmed:author
pubmed:issnType
Print
pubmed:volume
150
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
667-73
pubmed:dateRevised
2010-9-10
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
No evidence of HTLV-I proviral integration in lymphoproliferative disorders associated with cutaneous T-cell lymphoma.
pubmed:affiliation
Department of Dermatology, Case Western Reserve University, Cleveland, Ohio, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.