Linked Life Data

9025989

Source:http://linkedlifedata.com/resource/pubmed/id/9025989

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1997-4-15
pubmed:abstractText
DNA aneuploidy, which reflects changes in nuclear DNA-content, as determined cytometrically is a candidate prognostic factor in squamous cell carcinoma of the head and neck. The aim of this study was to compare two different preparation methods with respect to their use in detecting aneuploid tumor cell populations in malignant tumors of the head and neck. Fresh frozen tumor material was used. The methods compared were a multistep procedure (A) including fixation of cells and enzymatic treatment, and a one step procedure (B). Both include RNAse and the use of propidium iodide for DNA staining. Forty-seven percent of the tumors were non-diploid according to method A, and 29% according to method B, discordant findings being made in 15 tumors, only two of which were non-SCC. Defining a 'true non-diploid tumor' in this series as a tumor with a DNA index outside the range of diploidy detected either by method A or B, 59% (29/49) of the tumors were non-diploid. The sensitivity was 0.48 (14/29) for method B and 0.79 (23/29) for method A. The striking differences in accuracy between methods A and B emphasize the need of caution when new methods are introduced, and when results obtained with different methods are compared.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0921-8912
pubmed:author
pubmed:issnType
Print
pubmed:volume
12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
125-36
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Flow cytometry analysis of malignant tumors of the head and neck--differences between two methods in the recognition of aneuploidy.
pubmed:affiliation
Department of Otorhinolaryngology/Head and Neck Surgery, University Hospital, Lund, Sweden.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't