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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1-2
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pubmed:dateCreated |
1997-2-11
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pubmed:abstractText |
The neuroblastoma x glioma hybrid cell line NH15-CA2 was used to test the effects of two interleukins on neuronal Na+ channels. The cells were cultured in the presence of dibutyryl cAMP and retinoic acid, which yielded a high expression of Na+ channels so that the cells were excitable. Na+ currents were triggered and recorded in the whole-cell recording mode. Comparison of the effects of tetrodotoxin and mu-conotoxin established that the expressed Na+ channels were of the neuronal type. Bath-applied recombinant human interleukin-2 (rIL-2) had a reversible inhibitory effect on the Na+ currents, although to a lesser extent than on muscular Na+ currents (more than 1000 U/ml required for 50% block in NH15-CA2 cells vs 500 U/ml in myoballs). The current/voltage relationship was not affected by the presence of rIL-2, but the steady-state inactivation curve was shifted by -7.7 +/- 4.8 mV (mean +/- SD, n=18). Recombinant human interleukin-1beta (rIL-1beta), applied at 1000 U/ml, showed an inhibitory effect on the Na+ currents in about one-third of the cells tested. The mechanism of inhibition was different from that of rIL-2, as rIL-1beta seemed to cause a block without affecting voltage dependence or kinetics of the channels.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
0031-6768
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
433
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
160-5
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9019718-Animals,
pubmed-meshheading:9019718-Electric Conductivity,
pubmed-meshheading:9019718-Homeostasis,
pubmed-meshheading:9019718-Hybrid Cells,
pubmed-meshheading:9019718-Interleukin-1,
pubmed-meshheading:9019718-Interleukin-2,
pubmed-meshheading:9019718-Mice,
pubmed-meshheading:9019718-Neurons,
pubmed-meshheading:9019718-Rats,
pubmed-meshheading:9019718-Recombinant Proteins,
pubmed-meshheading:9019718-Sodium Channels,
pubmed-meshheading:9019718-Tumor Cells, Cultured
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pubmed:articleTitle |
Excitatory sodium currents of NH15-CA2 neuroblastoma x glioma hybrid cells are differently affected by interleukin-2 and interleukin-1beta.
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pubmed:affiliation |
Abteilung für Allgemeine Physiologie der Universität Ulm, D-89069 Ulm, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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