Linked Life Data

9015384

Source:http://linkedlifedata.com/resource/pubmed/id/9015384

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1997-2-24
pubmed:abstractText
To examine whether the hepatic aldehyde oxidases of male and female mice, which exhibit sex-related differences in benzaldehyde oxidation, are qualitatively different, we purified the enzymes from untreated male and female ddy mice and testosterone-pretreated female mice by sequential chromatography of benzamidine-Sepharose 6B and DEAE-5PW columns and characterized the enzymes. Purified enzymes from untreated male and female mice and from testosterone-treated females gave a single protein band at M(r) 265K and M(r) 138K on native and SDS-polyacrylamide gradient gel electrophoresis, respectively. The susceptibilities of the enzymes from both sexes to inhibitors such as menadione, norharman, quinacrine, estradiol, and SKF 525-A were also indistinguishable. However, the K(m) values for benzaldehyde, p-dimethylaminocinnamaldehyde, and 2-hydroxypyrimidine were two- to fourfold higher in the untreated female enzyme than in the untreated male enzyme, while the testosterone-induced female enzyme showed K(m) values similar to those of the male enzyme. These results indicate that the hepatic aldehyde oxidases of the sexes are quite similar with respect to molecular size and susceptibility to inhibitors, but their kinetic properties are somewhat different, suggesting the existence of microheterogeneity in sex differences. It also suggests that treatment of female mice with testosterone might induce the male-type enzyme.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0003-9861
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
338
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
29-34
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Purification and characterization of hepatic aldehyde oxidase in male and female mice.
pubmed:affiliation
Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine, Kasumi, Minami-ku, Japan. yosihara@pharm.hiroshima-u.ac.jp
pubmed:publicationType
Journal Article, Comparative Study