Linked Life Data

9013930

Source:http://linkedlifedata.com/resource/pubmed/id/9013930

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1997-3-4
pubmed:abstractText
nautilus (nau), one member of the myogenic regulatory family of bHLH-encoding genes, is expressed in a subset of muscle precursors and differentiated fibers in the Drosophila embryo. To elucidate the role of nautilus in myogenesis, we have misexpressed it using the GAL4-targeted system. We find that ectopic expression results in lethality throughout Drosophila development. We analyzed the effects of embryonic expression in mesodermal tissues that include the cardioblasts of the dorsal vessel as well most, if not all, of the presumptive somatic muscle precursors. Immunohistochemical staining for muscle MHC revealed abnormalities that include an absence of cardial cells, coincident with the appearance of novel muscle fibers adjacent to the dorsal vessel. Moreover, many cardioblasts express increased levels of muscle-specific genes such as myosin, actin 57B, and Mlp60A, a protein that is restricted to the somatic, visceral, and pharyngeal muscles. These data suggest that the missing cardial cells have been transformed into cells with properties similar to those of the somatic muscles. In addition, ubiquitous expression of nautilus in somatic muscle cells of these embryos resulted in muscle pattern defects. Specifically, muscles that do not normally express nautilus were frequently absent, and novel fibers were observed in positions reminiscent of nau-expressing muscles. These data imply that nautilus can alter the developmental program of muscle precursors. In summary, we suggest that nautilus induces myogenic differentiation in vivo when ectopically expressed in developing cardioblasts and may affect the myogenic differentiation program of specific muscle fibers.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0012-1606
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
181
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
197-212
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:9013930-Animals, pubmed-meshheading:9013930-Basic Helix-Loop-Helix Transcription Factors, pubmed-meshheading:9013930-Cell Differentiation, pubmed-meshheading:9013930-DNA-Binding Proteins, pubmed-meshheading:9013930-Drosophila Proteins, pubmed-meshheading:9013930-Drosophila melanogaster, pubmed-meshheading:9013930-Embryo, Nonmammalian, pubmed-meshheading:9013930-Gene Expression Regulation, Developmental, pubmed-meshheading:9013930-Insect Proteins, pubmed-meshheading:9013930-Mesoderm, pubmed-meshheading:9013930-Morphogenesis, pubmed-meshheading:9013930-Multigene Family, pubmed-meshheading:9013930-Muscle Fibers, Skeletal, pubmed-meshheading:9013930-Muscle Proteins, pubmed-meshheading:9013930-Myocardium, pubmed-meshheading:9013930-Pupa, pubmed-meshheading:9013930-Recombinant Fusion Proteins, pubmed-meshheading:9013930-Transcription Factors, pubmed-meshheading:9013930-Transfection
pubmed:year
1997
pubmed:articleTitle
Misexpression of nautilus induces myogenesis in cardioblasts and alters the pattern of somatic muscle fibers.
pubmed:affiliation
Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park 16802, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't