Linked Life Data

9010674

Source:http://linkedlifedata.com/resource/pubmed/id/9010674

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1997-3-26
pubmed:abstractText
The effects of recombinant human interferon alpha (rhIFN-alpha) and interferon gamma (rhIFN-gamma) were examined on the apoptosis of human cord blood derived eosinophils, obtained after 4 weeks of culture with recombinant human interleukin-3 (rhIL-3), granulocyte-macrophage-colony stimulating factor (rhGM-CSF) and interleukin-5 (rhIL-5). Eosinophil viability decreased remarkably after 1 week culture with rhIFN-alpha and rhIFN-gamma. Recombinant rhIFN-alpha also decreased the viability of co-existing monocytes/macrophages, whereas in contrast, rhIFN-gamma increased the percentage of viable monocytes/macrophages. There was no synergistic or additional effect of rhIFN-alpha and rhIFN-gamma on eosinophil viability. Apoptotic eosinophils, detected by their morphological characteristics, or by DNA nick end labeling in situ, increased remarkably after incubation with rhIFN-alpha and increased to a lesser extent with rhIFN-gamma. The numbers of eosinophil-phagocytosing macrophages increased after culture with rhIFN-alpha and also with rhIFN-gamma. In contrast, eosinophilopoietic cytokines such as rhIL-3, rhIL-5 and specially rhGM-CSF, significantly increased eosinophil viability, and partially rescued the effects of rhIFNs. They also decreased apoptotic eosinophil numbers and eosinophil-phagocytosing macrophage numbers. These results indicate that eosinophil viability, at least in vitro, can be differentially regulated by cytokines produced during the immune response.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1148-5493
pubmed:author
pubmed:issnType
Print
pubmed:volume
7
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
725-32
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Induction by interferons of human eosinophil apoptosis and regulation by interleukin-3, granulocyte/macrophage-colony stimulating factor and interleukin-5.
pubmed:affiliation
Centre d'Immunologie et de Biologie Parasitaire, Unite INSERM U167, Institut Pasteur, Lille, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't