Linked Life Data

9010181

Source:http://linkedlifedata.com/resource/pubmed/id/9010181

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1997-2-24
pubmed:abstractText
Our goal in this study was to evaluate the effects of and the interaction between the hypothesized principal determinants of the incorporation of grafts: antigenicity and treatment of the graft. We implanted fresh and frozen cortical bone grafts that were matched for both major and non-major histocompatibility complex antigens (syngeneic grafts), matched for major but not for non-major histocompatibility complex antigens (minor mismatch), and mismatched for both major and non-major histocompatibility complex antigens (major mismatch). We used a rat model with an eight-millimeter segmental defect in the femur. The construct was stabilized with a plastic plate, threaded Kirschner wires, and cerclage wires. We evaluated the grafts at one, two, and four months after implantation. We measured the immune response; assessed the incorporation of the graft with use of histological examination, biomechanical testing, and quantitative isotopic kinetics; and statistically analyzed the effects of and the interactions among three independent variables: time, the degree of matching for major histocompatibility complex antigens, and the treatment of the graft (whether it was fresh or frozen). These three independent variables had profound effects on the pattern, rate, and quality of the incorporation of the graft. Two-way and three-way interactions among these variables were also noted. Serial changes in every dependent variable were observed with time. Systemic antibody specific for donor antigens was measurable only in the serum of animals that had a major mismatch, but freezing markedly attenuated the systemic antibody response. Revascularization was profoundly affected by histocompatibility-antigen matching; the syngeneic grafts were revascularized more quickly and to a greater degree than the grafts with either a minor or a major mismatch. Freezing significantly (p < 0.001) reduced the revascularization of the syngeneic grafts but had no discernible effect on the grafts with a minor mismatch.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0021-9355
pubmed:author
pubmed:issnType
Print
pubmed:volume
79
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1-16
pubmed:dateRevised
2010-10-25
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Critical biological determinants of incorporation of non-vascularized cortical bone grafts. Quantification of a complex process and structure.
pubmed:affiliation
Department of Orthopaedics, Case Western Reserve University, Cleveland, Ohio 44106, USA.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S.