Linked Life Data

9007037

Source:http://linkedlifedata.com/resource/pubmed/id/9007037

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1997-2-24
pubmed:abstractText
The toxicity of allyl alcohol was compared in freshly isolated and cryopreserved hepatocytes that were either placed in suspension or maintained on hydrated collagen gels in a sandwich configuration. The purpose of this study was to evaluate whether the two types of cells displayed the same sensitivity to allyl alcohol when maintained in vitro over relatively prolonged periods of time. The important differentiated functions of urea synthesis, secretion of albumin, and metabolism of ethoxycoumarin, a model drug substrate, were used as end points of toxicity. Cryopreserved hepatocytes incubated in physiological buffer shortly after removal from liquid nitrogen were more sensitive to allyl alcohol than freshly isolated hepatocytes. In contrast, cryopreserved and freshly isolated hepatocytes maintained on hydrated collagen gels responded identically to allyl alcohol. Thus, the increased sensitivity of cryopreserved hepatocytes in suspension to allyl alcohol is a transient phenomenon that disappears after the cells have been allowed to recover on hydrated collagen gels. Dissipation of the mitochondrial membrane potential by allyl alcohol, as indexed by rhodamine 123 fluorescence, was also the same in freshly isolated and cryopreserved hepatocytes maintained on hydrated collagen matrices. This loss of mitochondrial membrane potential caused by allyl alcohol preceded inhibition of albumin and urea biosynthesis. Collectively, the results indicate that cryopreserved cells maintained on hydrated collagen gels provide a useful system to define the actions of certain hepatotoxic agents over relatively prolonged periods of time in vitro.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0041-008X
pubmed:author
pubmed:issnType
Print
pubmed:volume
142
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
87-94
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Toxicity of allyl alcohol in primary cultures of freshly isolated and cryopreserved hepatocytes maintained on hydrated collagen gels.
pubmed:affiliation
Laboratory for Cellular and Biochemical Toxicology, Department of Pharmacology and Toxicology, Rutgers University, Piscataway, New Jersey 08854, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't