Linked Life Data

8997507

Source:http://linkedlifedata.com/resource/pubmed/id/8997507

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1997-4-15
pubmed:abstractText
The mRNA encoding the alpha 1 subunit of soluble guanylyl cyclase (alpha 1sGC) was identified in a different-display screening for genes spatially and temporally regulated during the development of fetal rat brain. The initially isolated fragment of the 3' untranslated region was used for in situ hybridization and to produce full-length cDNA clones by hybridization screening of cDNA libraries and by RACE (rapid amplification of cDNA ends), respectively. In situ hybridization analysis that alpha 1sGC was absent at embryonic day 12 (E12), but by E14-E15, the forebrain exhibited dense expression in the developing striatum, medial cerebral wall containing the presumptive hippocampus, cerebellar neuroepithelium, and roof plate. Weaker expression was observed in the septum, epithalamus, ventral thalamus, pineal gland and retina. This pattern is largely maintained and refined at E18, with additional expression domains in the olfactory tubercle, nucleus accumbens, zona incerta and neocortex. During early postnatal development, the adult pattern is expressed, as previously reported. The unexpected, early expression of alpha 1sGC, in conjunction with the known absence of its heterodimeric partner, the beta subunit of sGC, from the developing rodent brain during fetal ages raises potentially novel functional roles of the alpha 1 subunit during ontogeny, and might imply the existence of an alternative beta subunit specific for the prenatal brain.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0165-3806
pubmed:author
pubmed:issnType
Print
pubmed:day
23
pubmed:volume
97
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
226-34
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
The alpha 1 subunit of soluble guanylyl cyclase is expressed prenatally in the rat brain.
pubmed:affiliation
Department of Neuroscience and Cell Biology, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, Piscataway 08854, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.