Linked Life Data

8997243

Source:http://linkedlifedata.com/resource/pubmed/id/8997243

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6 Pt 1
pubmed:dateCreated
1997-2-12
pubmed:abstractText
Human hepatocarcinoma-intestine-pancreas (HIP) cDNA, isolated from a hepatocellular carcinoma, encodes a C-type lectin. According to published cDNA sequences, HIP protein is identical to human pancreatitis-associated protein (PAP). In these sequences, a putative signal peptide and the carbohydrate recognition domain (CRD) can be recognized. In the present study, we established transgenic mice to drive the production of soluble recombinant HIP/PAP protein in the milk of lactating animals; using this model, we showed that HIP/PAP protein was secreted after suitable cleavage of the potential signal peptide. Moreover, we also produced HIP/PAP protein by Escherichia coli cultures performed to generate specific antibodies. These antibodies enabled the detection of HIP/PAP protein in normal intestine and pancreas (both in endocrine and exocrine cells), e.g., intestinal neuroendocrine and Paneth cells, pancreatic islets of Langerhans, and acinar cells. HIP/PAP protein was also identified in the cytoplasm of tumoral hepatocytes but not in nontumoral hepatocytes. Finally, HIP/PAP protein activity was tested and we showed that HIP/PAP induced the adhesion of rat hepatocytes and bound strongly to extracellular matrix proteins (laminin-1, fibronectin), less strongly to type I and IV collagen, and not at all to heparan sulfate proteoglycan. In conclusion, these results showed that HIP/PAP protein was matured on secretion. We also demonstrated that HIP/PAP protein was specifically expressed in hepatocarcinoma cells and interacted with rat hepatocytes and the extracellular matrix. Taken overall, these results suggest that HIP/PAP protein may be of potential importance to liver cell differentiation/proliferation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0002-9513
pubmed:author
pubmed:issnType
Print
pubmed:volume
271
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
G993-1002
pubmed:dateRevised
2010-2-8
pubmed:meshHeading
pubmed-meshheading:8997243-Acute-Phase Proteins, pubmed-meshheading:8997243-Animals, pubmed-meshheading:8997243-Antigens, Neoplasm, pubmed-meshheading:8997243-Carcinoma, Hepatocellular, pubmed-meshheading:8997243-Cell Adhesion, pubmed-meshheading:8997243-Cell Adhesion Molecules, pubmed-meshheading:8997243-Humans, pubmed-meshheading:8997243-Immunohistochemistry, pubmed-meshheading:8997243-Lectins, C-Type, pubmed-meshheading:8997243-Liver, pubmed-meshheading:8997243-Liver Neoplasms, pubmed-meshheading:8997243-Male, pubmed-meshheading:8997243-Mice, pubmed-meshheading:8997243-Mice, Transgenic, pubmed-meshheading:8997243-Pancreas, pubmed-meshheading:8997243-Proteins, pubmed-meshheading:8997243-Rats, pubmed-meshheading:8997243-Rats, Sprague-Dawley, pubmed-meshheading:8997243-Recombinant Proteins, pubmed-meshheading:8997243-Tumor Cells, Cultured, pubmed-meshheading:8997243-Tumor Markers, Biological
pubmed:year
1996
pubmed:articleTitle
HIP/PAP is an adhesive protein expressed in hepatocarcinoma, normal Paneth, and pancreatic cells.
pubmed:affiliation
Institut National de la Santé et de la Recherche Médicale Unité 370, Centre Hospitalier Universitaire Necker, Paris, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't