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rdf:type |
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lifeskim:mentions |
umls-concept:C0007589,
umls-concept:C0017262,
umls-concept:C0026473,
umls-concept:C0040648,
umls-concept:C0185117,
umls-concept:C0205145,
umls-concept:C0205314,
umls-concept:C0379099,
umls-concept:C0679622,
umls-concept:C0851285,
umls-concept:C0919496,
umls-concept:C0968902,
umls-concept:C1511938,
umls-concept:C1705552,
umls-concept:C1711351,
umls-concept:C2911684
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pubmed:issue |
1
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pubmed:dateCreated |
1997-2-18
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pubmed:abstractText |
During monocyte-to-macrophage differentiation, the cellular content of vacuolar H+-ATPase (V-ATPase) increases more than 4-fold. We have shown previously that amplified expression of the B2 subunit of the V-ATPase occurs solely by increased transcription, and that the 5'-untranslated region of the B2 gene, containing multiple consensus binding sites for the transcription factors AP-2 and Sp1, is required for this expression. The present study demonstrates that AP-2 binding sequences are essential for increased transcription from the B2 promoter during monocyte-macrophage differentiation and that AP-2, expressed exogenously in THP-1 and other cells, activates transcription from the B2 promoter. In mobility shift assays, a nuclear factor from THP-1 and U-937 cells was identified that binds to several AP-2 response elements within the B2 promoter, but does not react with AP-2 antibodies, and has a DNA sequence binding affinity profile that differs from AP-2. These findings suggest that a novel AP-2-like transcription factor is responsible for V-ATPase B subunit amplification during monocyte differentiation.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
3
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pubmed:volume |
272
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
174-81
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:8995244-Base Sequence,
pubmed-meshheading:8995244-Binding Sites,
pubmed-meshheading:8995244-Cell Differentiation,
pubmed-meshheading:8995244-Cell Line,
pubmed-meshheading:8995244-DNA,
pubmed-meshheading:8995244-DNA-Binding Proteins,
pubmed-meshheading:8995244-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:8995244-Humans,
pubmed-meshheading:8995244-Isoenzymes,
pubmed-meshheading:8995244-Macrophages,
pubmed-meshheading:8995244-Molecular Sequence Data,
pubmed-meshheading:8995244-Monocytes,
pubmed-meshheading:8995244-Nuclear Proteins,
pubmed-meshheading:8995244-Promoter Regions, Genetic,
pubmed-meshheading:8995244-Proton-Translocating ATPases,
pubmed-meshheading:8995244-Sp1 Transcription Factor,
pubmed-meshheading:8995244-Transcription Factor AP-2,
pubmed-meshheading:8995244-Transcription Factors,
pubmed-meshheading:8995244-Vacuolar Proton-Translocating ATPases
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pubmed:year |
1997
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pubmed:articleTitle |
A novel transcription factor regulates expression of the vacuolar H+-ATPase B2 subunit through AP-2 sites during monocytic differentiation.
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pubmed:affiliation |
Department of Medicine/Renal Division, George M. O'Brien Center for Kidney and Urological Diseases, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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