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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
7
|
| pubmed:dateCreated |
1997-1-16
|
| pubmed:abstractText |
A new restriction endonuclease was isolated from the Bacillus cereus BKM B-814 by means of the cell disruption with ultrasonication, ammonium sulfate fractionation of the cell-free extract, and chromatography on DEAE-Sepharose to give about 1400 U of the enzyme per gram of cells. The enzyme revealed the maximum activity at 30-37 degrees C, pH 7.6-8.2, and 5-10 mM MgCl2 under a high ionic strength (50 mM Tris-HCl, 100 mM NaCl). The site-specific endonuclease BcuAI was found to recognize the 5' G decreases G(A/T)CC sequence in double-stranded DNA and cleave it as shown with the arrow, thus being a true isoschisomer of the AvaII restriction endonuclease.
|
| pubmed:language |
rus
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0132-3423
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
22
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
528-31
|
| pubmed:dateRevised |
2008-8-23
|
| pubmed:meshHeading |
pubmed-meshheading:8992958-Bacillus cereus,
pubmed-meshheading:8992958-Chromatography, Ion Exchange,
pubmed-meshheading:8992958-DNA, Recombinant,
pubmed-meshheading:8992958-Deoxyribonucleases, Type II Site-Specific,
pubmed-meshheading:8992958-Electrophoresis, Agar Gel,
pubmed-meshheading:8992958-Hydrolysis,
pubmed-meshheading:8992958-Substrate Specificity
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
[Site-specific BcuAI endonuclease from Bacillus cereus A].
|
| pubmed:publicationType |
Journal Article,
English Abstract
|