Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1997-1-30
|
| pubmed:abstractText |
A sandwich-type enzyme immunoassay has been developed for measuring human big endothelin-1 (big ET-1) in human plasma and supernatant fluids from human cell cultures. Big ET-1 is the precursor of endothelin 1 (ET-1), the most potent vasoconstrictor known. A rabbit antibody raised against the big ET-1 COOH-terminus fragment was used as an immobilized antibody (anti-P16). The Fab' fragment of a monoclonal antibody (1B3) raised against the ET-1 loop fragment was used as the enzyme-labeled antibody, after being coupled to acetylcholinesterase. The lowest detectable value in the assay was 1.2 pg/mL (0.12 pg/well). The assay was highly specific for big ET-1, demonstrating no cross-reactivity with ET-1, <0.4% cross-reactivity with big endothelin-2 (big ET-2), and <0.1% with big endothelin-3 (big ET-3). We used this assay to evaluate the effect of two different postural positions (supine and standing) on plasma big ET-1 concentrations in 11 male and 11 female healthy subjects. Data analysis revealed that neither sex nor body position influenced plasma big ET-1 concentrations. This assay should thus permit the detection of possible variations in plasma concentrations of big ET-1 in certain pathologies and, in association with ET-1 assay, make possible in vitro study of endothelin-converting enzyme activity in cell models. Such studies could clarify the physiological and clinical roles of this family of peptides.
|
| pubmed:commentsCorrections | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Endothelin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Endothelins,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Fab Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Precursors
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0009-9147
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
43
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
64-70
|
| pubmed:dateRevised |
2005-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:8990224-Adolescent,
pubmed-meshheading:8990224-Adult,
pubmed-meshheading:8990224-Amino Acid Sequence,
pubmed-meshheading:8990224-Antibodies, Monoclonal,
pubmed-meshheading:8990224-Endothelin-1,
pubmed-meshheading:8990224-Endothelins,
pubmed-meshheading:8990224-Female,
pubmed-meshheading:8990224-Humans,
pubmed-meshheading:8990224-Immunoenzyme Techniques,
pubmed-meshheading:8990224-Immunoglobulin Fab Fragments,
pubmed-meshheading:8990224-Male,
pubmed-meshheading:8990224-Molecular Sequence Data,
pubmed-meshheading:8990224-Peptide Fragments,
pubmed-meshheading:8990224-Posture,
pubmed-meshheading:8990224-Protein Precursors,
pubmed-meshheading:8990224-Reference Values,
pubmed-meshheading:8990224-Reproducibility of Results,
pubmed-meshheading:8990224-Sensitivity and Specificity,
pubmed-meshheading:8990224-Sex Characteristics
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Sandwich-type enzyme immunoassay for big endothelin-I in plasma: concentrations in healthy human subjects unaffected by sex or posture.
|
| pubmed:affiliation |
Laboratoire de Biologie Hormonale, Hôpital Saint-Louis, Paris, France.
|
| pubmed:publicationType |
Journal Article
|