Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1997-1-27
pubmed:abstractText
This murine model of nontypeable (unencapsulated) Haemophilus influenzae (NTHI) bronchopneumonia used organisms bound to mouse fetal lung (MFL) cells as an inoculum. Pretreatment of the mice with 40 microL of 1% formalin 3 days before intranasal instillation of the bacteria was necessary to allow infection. The number of NTHI recovered from the lungs plus trachea on day 7 after instillation was >100 times the number originally inoculated. Later, however, the number of recovered bacteria diminished gradually, and by day 14 it was almost identical to the original inoculum size. Serum IgM also peaked on day 7 after infection, after which IgG increased while IgM decreased. Histologically, bronchoalveolar infiltration of neutrophils was observed on day 3 after inoculation and continued at least for the following 4 days. The present experiment demonstrates that MFL cells can protect bacteria that have invaded the cells from the opsonizing and killing activities of host humoral defense mechanisms.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0022-1899
pubmed:author
pubmed:issnType
Print
pubmed:volume
175
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
205-9
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed-meshheading:8985222-Animals, pubmed-meshheading:8985222-Anti-Bacterial Agents, pubmed-meshheading:8985222-Bacterial Adhesion, pubmed-meshheading:8985222-Blood Bactericidal Activity, pubmed-meshheading:8985222-Bronchi, pubmed-meshheading:8985222-Bronchopneumonia, pubmed-meshheading:8985222-Cells, Cultured, pubmed-meshheading:8985222-Colony Count, Microbial, pubmed-meshheading:8985222-Disease Models, Animal, pubmed-meshheading:8985222-Gentamicins, pubmed-meshheading:8985222-Haemophilus Infections, pubmed-meshheading:8985222-Haemophilus influenzae, pubmed-meshheading:8985222-Immunoglobulin M, pubmed-meshheading:8985222-Lung, pubmed-meshheading:8985222-Male, pubmed-meshheading:8985222-Mice, pubmed-meshheading:8985222-Mice, Inbred ICR, pubmed-meshheading:8985222-Pneumonia, Bacterial, pubmed-meshheading:8985222-Pulmonary Alveoli, pubmed-meshheading:8985222-Trachea
pubmed:year
1997
pubmed:articleTitle
New murine model of bronchopneumonia due to cell-bound Haemophilus influenzae.
pubmed:affiliation
Department of Microbiology, School of Medicine, Toho University, Tokyo, Japan.
pubmed:publicationType
Journal Article