Linked Life Data

8980008

Source:http://linkedlifedata.com/resource/pubmed/id/8980008

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1997-10-2
pubmed:abstractText
A replication-defective adenovirus vector carrying the reporter gene encoding beta-galactosidase was used to transfect organotypic slices maintained in culture for up to 1 month. Three different delivery systems were used to inoculate the viral solution, either into the culture medium, or directly onto the surface of the slices or by microinjection into the tissue. Using the two first paradigms beta-galactosidase expressing cells were mostly of glial phenotype and distributed throughout the slices without any specific regional pattern. In contrast, microinjection of the adenovirus resulted in a large number of both infected neurones and glia, concentrated at the site of injection. This method thus appears to be able to circumvent some of the constraints and limitations associated with in vivo gene transfer.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0969-9961
pubmed:author
pubmed:copyrightInfo
Copyright 1995 Academic Press, Inc.
pubmed:issnType
Print
pubmed:volume
2
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
49-54
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Adenovirus mediated gene transfer in organotypic brain slices.
pubmed:affiliation
Institut Alfred Fessard, Centre National de la Recherche Scientifique (CNRS), Gif sur Yvette, 91198, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't