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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
1997-2-5
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pubmed:abstractText |
A recombinant baculovirus was constructed to express a cDNA encoding RelA (p65), a member of the NF-kappa B/Rel family of proteins. Infection of Spodoptera frugiderda insect cells with the recombinant baculovirus resulted in the production of the biologically active protein as measured by immunoblotting using RelA-specific antisera and by electrophoretic mobility shift assays. The recombinant protein bound specifically to an oligonucleotide containing the NF-kappa B consensus motif but not to that containing the unrelated Oct-1 consensus motif. Thus insect cell-derived RelA possess properties similar to the native protein and may be used in physical, biochemical, and pharmacological studies.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
1065-6995
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
20
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
777-9
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pubmed:dateRevised |
2006-4-21
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pubmed:meshHeading |
pubmed-meshheading:8979371-Animals,
pubmed-meshheading:8979371-Binding Sites,
pubmed-meshheading:8979371-Cell Line,
pubmed-meshheading:8979371-Cloning, Molecular,
pubmed-meshheading:8979371-DNA,
pubmed-meshheading:8979371-DNA, Complementary,
pubmed-meshheading:8979371-Genetic Vectors,
pubmed-meshheading:8979371-Humans,
pubmed-meshheading:8979371-NF-kappa B,
pubmed-meshheading:8979371-Nucleopolyhedrovirus,
pubmed-meshheading:8979371-Recombinant Fusion Proteins,
pubmed-meshheading:8979371-Spodoptera,
pubmed-meshheading:8979371-Transcription Factor RelA
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pubmed:year |
1996
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pubmed:articleTitle |
Production of biologically active human RelA (p65) in baculovirus-infected insect cells.
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pubmed:affiliation |
Department of Immunopharmacology, SmithKline Beecham Pharmaceuticals, King of Prussia, PA 19406-0939, USA.
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pubmed:publicationType |
Journal Article
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