Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1997-1-27
pubmed:abstractText
The present study investigated the effects of extracellular ATP on the intracellular calcium ion concentration ([Ca2+]i) and testosterone production in isolated adult rat Leydig cells. This nucleotide caused an increase in [Ca2+]i, with a maximal effect at a concentration of 100 microM ATP, comprising a rapid initial spike followed by a long-lasting plateau. The first rapid spike was dependent on the release of Ca2+ from internal stores, since it also occurred in Ca(2+)-free medium and was abolished after depletion of internal stores with thapsigargin. The second, long-lasting, phase was dependent on the influx of Ca2+ from the extracellular medium. After 3 h of incubation, extracellular ATP stimulated testosterone secretion in a dose-dependent manner, with a maximal effect at 100 microM. Activation of steroidogenesis by ATP was fully dependent on the presence of Ca2+ in the external medium. Among different nucleotides, only ATP, adenosine 5'-[gamma-thio]triphosphate, UTP, benzoylbenzoic-ATP and 2-methylthio-ATP were effective in inducing both the rise in [Ca2+]i and testosterone secretion. These effects were blocked by preincubation of Leydig cells with oxidized ATP, an inhibitor of the P2Z-purinergic receptor subtype. These results show that rat Leydig cells possess P2-purinergic receptors whose activation triggers an increase in [Ca2+]i due to the release of Ca2+ from internal stores and Ca2+ influx from the external medium. The stimulatory effect of extracellular ATP on testosterone secretion seems to be coupled to the influx of Ca2+ from the external medium.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8973559-1325838, http://linkedlifedata.com/resource/pubmed/commentcorrection/8973559-1390294, http://linkedlifedata.com/resource/pubmed/commentcorrection/8973559-1423506, http://linkedlifedata.com/resource/pubmed/commentcorrection/8973559-1471458, http://linkedlifedata.com/resource/pubmed/commentcorrection/8973559-1527064, http://linkedlifedata.com/resource/pubmed/commentcorrection/8973559-171267, http://linkedlifedata.com/resource/pubmed/commentcorrection/8973559-1744994, http://linkedlifedata.com/resource/pubmed/commentcorrection/8973559-2001232, http://linkedlifedata.com/resource/pubmed/commentcorrection/8973559-2138778, http://linkedlifedata.com/resource/pubmed/commentcorrection/8973559-2152880, http://linkedlifedata.com/resource/pubmed/commentcorrection/8973559-2454675, http://linkedlifedata.com/resource/pubmed/commentcorrection/8973559-2464587, http://linkedlifedata.com/resource/pubmed/commentcorrection/8973559-2506191, http://linkedlifedata.com/resource/pubmed/commentcorrection/8973559-3024621, http://linkedlifedata.com/resource/pubmed/commentcorrection/8973559-3042486, http://linkedlifedata.com/resource/pubmed/commentcorrection/8973559-3532817, http://linkedlifedata.com/resource/pubmed/commentcorrection/8973559-4396222, http://linkedlifedata.com/resource/pubmed/commentcorrection/8973559-7523952, http://linkedlifedata.com/resource/pubmed/commentcorrection/8973559-7542932, http://linkedlifedata.com/resource/pubmed/commentcorrection/8973559-7575464, http://linkedlifedata.com/resource/pubmed/commentcorrection/8973559-7685114, http://linkedlifedata.com/resource/pubmed/commentcorrection/8973559-7850003, http://linkedlifedata.com/resource/pubmed/commentcorrection/8973559-8119196, http://linkedlifedata.com/resource/pubmed/commentcorrection/8973559-8126137, http://linkedlifedata.com/resource/pubmed/commentcorrection/8973559-8214015, http://linkedlifedata.com/resource/pubmed/commentcorrection/8973559-8391422, http://linkedlifedata.com/resource/pubmed/commentcorrection/8973559-8463330
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0264-6021
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
320 ( Pt 2)
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
499-504
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Role of P2-purinergic receptors in rat Leydig cell steroidogenesis.
pubmed:affiliation
Università di Padova, Italy.
pubmed:publicationType
Journal Article, In Vitro