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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1997-3-12
|
| pubmed:abstractText |
We characterized a murine monoclonal antibody, PT25-2 (IgG1), raised against washed human platelets. The antibody and its Fab fragments were both capable of inducing platelet aggregation in a fibrinogen-dependent manner and induced 125I-fibrinogen binding to unstimulated platelets (120,000 molecules/platelet at a 100 nM IgG concentration). The antibody immunoprecipitated the alpha IIb beta 3 complex from lysates of iodinated platelets but did not react with the respective subunits when complex formation was disrupted by treatment with 5 mM EDTA at 37 degrees C for 30 min. However, simply removing the extracellular divalent cation with EDTA had no effect on antibody binding indicating that the antibody's epitope depends upon a conformational structure maintained by alpha beta subunit association. Antibody binding to unstimulated, washed platelets yielded binding parameters (Kd = 40 nM, Bmax = 100,000 molecules/platelet), which were found to be virtually unchanged when binding was performed using thrombin or RGDS-peptide-stimulated platelets. Thus, the PT25-2 antibody defines a novel regulatory epitope expressed by the alpha IIb beta 3 integrin on unstimulated, quiescent platelets.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0340-6245
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
76
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1038-46
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:8972029-Animals,
pubmed-meshheading:8972029-Antibodies, Monoclonal,
pubmed-meshheading:8972029-Blood Platelets,
pubmed-meshheading:8972029-Epitope Mapping,
pubmed-meshheading:8972029-Flow Cytometry,
pubmed-meshheading:8972029-Humans,
pubmed-meshheading:8972029-Immunodominant Epitopes,
pubmed-meshheading:8972029-Mice,
pubmed-meshheading:8972029-Mice, Inbred BALB C,
pubmed-meshheading:8972029-Platelet Aggregation,
pubmed-meshheading:8972029-Platelet Glycoprotein GPIIb-IIIa Complex
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
A novel regulatory epitope defined by a murine monoclonal antibody to the platelet GPIIb-IIIa complex (alpha IIb beta 3 integrin).
|
| pubmed:affiliation |
Department of Internal Medicine, School of Medicine, Keio University, Tokyo, Japan.
|
| pubmed:publicationType |
Journal Article
|