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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1977-10-28
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pubmed:abstractText |
Analogues of ethidium chloride with large quaternary side chains have been synthesized and evaluated for improved resolution in the dye-buoyant density system for the separation of open and closed circular DNA. These compounds are similar to propidium which differs from ethidium by the replacement of an ethyl group by a methyldiethylaminopropyl group. The new analogues contain a triethylamino group attached to a methylene chain with 3, 5 or 7 carbons. With PM2 DNA the observed separation for propidium is 1.97 times that of ethidium and the new dyes show separations relative to ethidium of 2.27, 2.66 and 2.77. A correlation is established between the mass of the dye component and the observed separation which is retionalized on the basis of the four component thermodynamics describing this system.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/896463-1257050,
http://linkedlifedata.com/resource/pubmed/commentcorrection/896463-13712606,
http://linkedlifedata.com/resource/pubmed/commentcorrection/896463-4296517,
http://linkedlifedata.com/resource/pubmed/commentcorrection/896463-4308095,
http://linkedlifedata.com/resource/pubmed/commentcorrection/896463-4313852,
http://linkedlifedata.com/resource/pubmed/commentcorrection/896463-4321914,
http://linkedlifedata.com/resource/pubmed/commentcorrection/896463-4333613,
http://linkedlifedata.com/resource/pubmed/commentcorrection/896463-4944065,
http://linkedlifedata.com/resource/pubmed/commentcorrection/896463-4947800,
http://linkedlifedata.com/resource/pubmed/commentcorrection/896463-5231757,
http://linkedlifedata.com/resource/pubmed/commentcorrection/896463-5259643,
http://linkedlifedata.com/resource/pubmed/commentcorrection/896463-5260915,
http://linkedlifedata.com/resource/pubmed/commentcorrection/896463-5265062,
http://linkedlifedata.com/resource/pubmed/commentcorrection/896463-6082458
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
4
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1349-59
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:896463-Bacteriophages,
pubmed-meshheading:896463-Centrifugation, Density Gradient,
pubmed-meshheading:896463-DNA, Circular,
pubmed-meshheading:896463-DNA, Viral,
pubmed-meshheading:896463-Ethidium,
pubmed-meshheading:896463-Mathematics,
pubmed-meshheading:896463-Molecular Weight,
pubmed-meshheading:896463-Pseudomonas,
pubmed-meshheading:896463-Structure-Activity Relationship
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pubmed:year |
1977
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pubmed:articleTitle |
Ethidium analogues with improved resolution in the dye-buoyant density procedure.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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