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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1997-1-16
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pubmed:abstractText |
A relatively rapid protein phosphatase-based assay was developed for detecting okadaic acid in extracts of shellfish, using oysters (Crassostrea virginica) as a model. The assay has good sensitivity, detecting okadaic acid in crude methanolic extracts of oysters at > or = 4 ng/mL (> or = 0.1 ng/assay). Assay accuracy for detecting toxic shellfish was validated through a series of spike recovery experiments. In more than 320 assessments, all oysters containing toxic amounts of okadaic acid (> or = 0.2 microgram/g) were detected. Results of analysis of the same extract by phosphatase assay and liquid chromatography gave very high correlation.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1060-3271
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
79
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1336-43
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pubmed:dateRevised |
2008-3-17
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pubmed:meshHeading |
pubmed-meshheading:8946712-Animals,
pubmed-meshheading:8946712-Chromatography, Liquid,
pubmed-meshheading:8946712-Dinoflagellida,
pubmed-meshheading:8946712-Food Contamination,
pubmed-meshheading:8946712-Marine Toxins,
pubmed-meshheading:8946712-Methanol,
pubmed-meshheading:8946712-Okadaic Acid,
pubmed-meshheading:8946712-Ostreidae,
pubmed-meshheading:8946712-Phosphoprotein Phosphatases,
pubmed-meshheading:8946712-Pyrans,
pubmed-meshheading:8946712-Reproducibility of Results,
pubmed-meshheading:8946712-Serine
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pubmed:articleTitle |
Detection of DSP-toxins, okadaic acid, and dinophysis toxin-1 in shellfish by serine/threonine protein phosphatase assay.
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pubmed:affiliation |
University of South Alabama, College of Medicine, Department of Biochemistry and Molecular Biology, Mobile 36688, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, Non-P.H.S.
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