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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5 Pt 1
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pubmed:dateCreated |
1997-1-9
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pubmed:abstractText |
Chloride channels supply critical functions in epithelial cells throughout the body. Although function of the volume- and voltage-gated C1C-2 is uncertain, its wide tissue distribution of mRNA suggests C1C-2 has important housekeeping functions. This study's objective was to identify the extent of not only C1C-2 mRNA expression but also protein expression as a measure of the capacity for C1C-2 chloride secretion in epithelial tissues. Using quantitative ribonuclease protection assay, we found that C1C-2 mRNA transcripts were abundant in fetal and postnatal brain, fetal kidney, liver, intestine, and lung. In contrast to brain, C1C-2 mRNA transcripts were downregulated during late gestation in lung, kidney, and intestine. The lung expressed the least C1C-2 mRNA. Immunoblotting demonstrated similar tissue- and gestation-dependent variations in C1C-2 protein expression. To determine if there is a correlation between the sites of C1C-2 protein expression and cystic fibrosis transmembrane conductance regulator (CFTR), another epithelial chloride channel, a polyclonal COOH-terminal C1C-2 antibody and an anti-R domain CFTR anti-body were used. C1C-2 and CFTR were expressed in different sites in lung and kidney.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0002-9513
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
271
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
L829-37
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:8944727-Aging,
pubmed-meshheading:8944727-Animals,
pubmed-meshheading:8944727-Animals, Newborn,
pubmed-meshheading:8944727-Brain,
pubmed-meshheading:8944727-Chloride Channels,
pubmed-meshheading:8944727-Embryonic and Fetal Development,
pubmed-meshheading:8944727-Female,
pubmed-meshheading:8944727-Fetus,
pubmed-meshheading:8944727-Gene Expression Regulation, Developmental,
pubmed-meshheading:8944727-Gestational Age,
pubmed-meshheading:8944727-Immunohistochemistry,
pubmed-meshheading:8944727-Intestines,
pubmed-meshheading:8944727-Kidney,
pubmed-meshheading:8944727-Liver,
pubmed-meshheading:8944727-Lung,
pubmed-meshheading:8944727-Nerve Tissue Proteins,
pubmed-meshheading:8944727-Organ Specificity,
pubmed-meshheading:8944727-Pregnancy,
pubmed-meshheading:8944727-RNA, Messenger,
pubmed-meshheading:8944727-Rats,
pubmed-meshheading:8944727-Rats, Sprague-Dawley,
pubmed-meshheading:8944727-Transcription, Genetic
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pubmed:year |
1996
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pubmed:articleTitle |
Gestational and tissue-specific regulation of C1C-2 chloride channel expression.
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pubmed:affiliation |
Eudowood Division of Respiratory Sciences, Johns Hopkins Medical Institutions, Baltimore, Maryland 21287, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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