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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1997-3-13
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pubmed:databankReference |
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pubmed:abstractText |
A DNA clone which complemented an arg-14 mutation of Neurospora crassa was isolated by sib selection from a cosmid library (pMOcosX). Southern and restriction-fragment-length polymorphism (RFLP) analysis confirmed that the cloned DNA contained the arg-14 gene. The arg-14 gene was identified as the structural gene for acetylglutamate synthase by immunodepletion of enzyme activity with antibodies prepared against an arg-14 fusion protein and by the thermal instability of acetylglutamate synthase in a temperature-sensitive arg-14 mutant. The fungai acetylglutamate synthase has little sequence homology to its bacterial counterpart, unlike other arginine biosynthetic enzymes. Expression of the arg-14 gene is regulated by cross-pathway control similar to many amino acid biosynthetic genes. However, expression of acetylglutamate synthase occurs throughout the developmental growth cycle, unlike other arginine biosynthetic enzymes.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0950-382X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
22
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
545-54
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:8939437-Arginine,
pubmed-meshheading:8939437-Blotting, Northern,
pubmed-meshheading:8939437-Blotting, Southern,
pubmed-meshheading:8939437-Chromosome Mapping,
pubmed-meshheading:8939437-Cloning, Molecular,
pubmed-meshheading:8939437-Cosmids,
pubmed-meshheading:8939437-DNA, Complementary,
pubmed-meshheading:8939437-DNA Primers,
pubmed-meshheading:8939437-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:8939437-Gene Expression Regulation, Fungal,
pubmed-meshheading:8939437-Gene Library,
pubmed-meshheading:8939437-Genes, Fungal,
pubmed-meshheading:8939437-Glutamate Synthase,
pubmed-meshheading:8939437-Hot Temperature,
pubmed-meshheading:8939437-Mitochondria,
pubmed-meshheading:8939437-Molecular Sequence Data,
pubmed-meshheading:8939437-Neurospora crassa,
pubmed-meshheading:8939437-Polymorphism, Restriction Fragment Length,
pubmed-meshheading:8939437-Recombinant Fusion Proteins,
pubmed-meshheading:8939437-Sequence Analysis, DNA,
pubmed-meshheading:8939437-Sequence Homology, Amino Acid,
pubmed-meshheading:8939437-Transformation, Genetic
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pubmed:year |
1996
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pubmed:articleTitle |
Acetylglutamate synthase from Neurospora crassa: structure and regulation of expression.
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pubmed:affiliation |
Department of Chemistry and Biochemistry, University of California, Los Angeles 90095-1569, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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