Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1997-4-1
pubmed:abstractText
1. Using a guinea-pig model of allergic asthma, we investigated the role of nitric oxide (NO) in allergen-induced airway hyperreactivity after the early asthmatic reaction, by examining the effects of the NO-synthase inhibitor N omega-nitro-L-arginine methyl ester (L-NAME) on the responsiveness to methacholine and histamine of isolated perfused tracheae from unchallenged (control) animals and from animals 6 h after ovalbumin challenge. 2. All animals developed airway hyperreactivity to inhaled histamine at 6 h after ovalbumin challenge, with a mean 3.11 +/- 0.45 fold increase in sensitivity to the agonist (P < 0.001). 3. In perfused tracheal preparations from the ovalbumin-challenged guinea-pigs, the maximal responses (Emax) to methacholine and histamine were significantly enhanced compared to controls, both after intraluminal (IL) and extraluminal (EL) administration of the contractile agonists. In addition, a small but significant increase in the pD2 (-log10 EC50) for IL and EL methacholine and for IL histamine was observed. As a consequence, the delta pD2 (EL-IL) for histamine was slightly decreased from 1.67 +/- 0.13 to 1.23 +/- 0.14 (P < 0.05). However, the delta pD2 for methacholine was unchanged (1.85 +/- 0.11 and 1.77 +/- 0.12, respectively; NS). 4. Incubation of control tracheae with 100 microM L-NAME (IL) significantly enhanced the Emax for both IL and EL methacholine and histamine to approximately the same degree as observed after ovalbumin challenge, with no effect on the pD2 and delta pD2 for both agonists. On the contrary, L-NAME had no effect on Emax and pD2 values of tracheal preparations from ovalbumin-challenged guinea-pigs. 5. L-NAME (10 microM-1 mM) had no effect on methacholine-induced contraction of isolated tracheal strip preparations obtained from control animals, indicating that L-NAME has no antimuscarinic effect on tracheal smooth muscle. 6. Histological examination of the intact tracheal preparations indicated epithelial and subepithelial infiltration of eosinophils after ovalbumin challenge. However, no apparent damage of the airway epithelium was observed in these preparations. 7. The results indicate that a deficiency of NO contributes to allergen-induced airway hyperreactivity after the early asthmatic reaction and that this deficiency appears not to be due to epithelial shedding.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8937712-1477482, http://linkedlifedata.com/resource/pubmed/commentcorrection/8937712-1644915, http://linkedlifedata.com/resource/pubmed/commentcorrection/8937712-1859060, http://linkedlifedata.com/resource/pubmed/commentcorrection/8937712-2200318, http://linkedlifedata.com/resource/pubmed/commentcorrection/8937712-2272372, http://linkedlifedata.com/resource/pubmed/commentcorrection/8937712-2565667, http://linkedlifedata.com/resource/pubmed/commentcorrection/8937712-2732146, http://linkedlifedata.com/resource/pubmed/commentcorrection/8937712-3192861, http://linkedlifedata.com/resource/pubmed/commentcorrection/8937712-3202421, http://linkedlifedata.com/resource/pubmed/commentcorrection/8937712-3286717, http://linkedlifedata.com/resource/pubmed/commentcorrection/8937712-3356665, http://linkedlifedata.com/resource/pubmed/commentcorrection/8937712-3514163, http://linkedlifedata.com/resource/pubmed/commentcorrection/8937712-3717766, http://linkedlifedata.com/resource/pubmed/commentcorrection/8937712-3977174, http://linkedlifedata.com/resource/pubmed/commentcorrection/8937712-3994155, http://linkedlifedata.com/resource/pubmed/commentcorrection/8937712-5000940, http://linkedlifedata.com/resource/pubmed/commentcorrection/8937712-589783, http://linkedlifedata.com/resource/pubmed/commentcorrection/8937712-7108068, http://linkedlifedata.com/resource/pubmed/commentcorrection/8937712-7504773, http://linkedlifedata.com/resource/pubmed/commentcorrection/8937712-7508323, http://linkedlifedata.com/resource/pubmed/commentcorrection/8937712-7509393, http://linkedlifedata.com/resource/pubmed/commentcorrection/8937712-7520003, http://linkedlifedata.com/resource/pubmed/commentcorrection/8937712-7524082, http://linkedlifedata.com/resource/pubmed/commentcorrection/8937712-7527428, http://linkedlifedata.com/resource/pubmed/commentcorrection/8937712-7663801, http://linkedlifedata.com/resource/pubmed/commentcorrection/8937712-7678206, http://linkedlifedata.com/resource/pubmed/commentcorrection/8937712-7682679, http://linkedlifedata.com/resource/pubmed/commentcorrection/8937712-7691109, http://linkedlifedata.com/resource/pubmed/commentcorrection/8937712-7767537, http://linkedlifedata.com/resource/pubmed/commentcorrection/8937712-7773550, http://linkedlifedata.com/resource/pubmed/commentcorrection/8937712-7889430, http://linkedlifedata.com/resource/pubmed/commentcorrection/8937712-7903007, http://linkedlifedata.com/resource/pubmed/commentcorrection/8937712-7904001, http://linkedlifedata.com/resource/pubmed/commentcorrection/8937712-8004304, http://linkedlifedata.com/resource/pubmed/commentcorrection/8937712-8006307, http://linkedlifedata.com/resource/pubmed/commentcorrection/8937712-8023911, http://linkedlifedata.com/resource/pubmed/commentcorrection/8937712-8037746, http://linkedlifedata.com/resource/pubmed/commentcorrection/8937712-8082696, http://linkedlifedata.com/resource/pubmed/commentcorrection/8937712-8368646, http://linkedlifedata.com/resource/pubmed/commentcorrection/8937712-8378262, http://linkedlifedata.com/resource/pubmed/commentcorrection/8937712-8406290
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0007-1188
pubmed:author
pubmed:issnType
Print
pubmed:volume
119
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1109-16
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Deficiency of nitric oxide in allergen-induced airway hyperreactivity to contractile agonists after the early asthmatic reaction: an ex vivo study.
pubmed:affiliation
Department of Medicinal Chemistry and Molecular Pharmacology, University Centre for Pharmacy, Groningen, The Netherlands.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't