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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
17
|
| pubmed:dateCreated |
1977-10-20
|
| pubmed:abstractText |
Zinc chelated by iminodiacetic acid linked to an insoluble matrix binds human fibroblast interferon quite selectively at neutral pH in 0.15 M NaCL. On reduction of the pH and increase of the ionic strength, the interferon is eluted. Using a pH gradient at constant high ionic strength, good purification of the interferon can be obtained, up to a final specific activity of 108.5 units/mg of protein. Approximately 60% of the applied activity can be recovered.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0021-9258
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
10
|
| pubmed:volume |
252
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
5934-5
|
| pubmed:dateRevised |
2009-10-27
|
| pubmed:meshHeading |
pubmed-meshheading:893388-Cells, Cultured,
pubmed-meshheading:893388-Chromatography, Affinity,
pubmed-meshheading:893388-Diploidy,
pubmed-meshheading:893388-Fibroblasts,
pubmed-meshheading:893388-Humans,
pubmed-meshheading:893388-Interferons,
pubmed-meshheading:893388-Leukocytes,
pubmed-meshheading:893388-Lymphocytes,
pubmed-meshheading:893388-Zinc
|
| pubmed:year |
1977
|
| pubmed:articleTitle |
Purification of human fibroblast interferon by zinc chelate affinity chromatography.
|
| pubmed:publicationType |
Journal Article
|