| pubmed-article:8933368 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8933368 | lifeskim:mentions | umls-concept:C0010453 | lld:lifeskim |
| pubmed-article:8933368 | lifeskim:mentions | umls-concept:C0025914 | lld:lifeskim |
| pubmed-article:8933368 | lifeskim:mentions | umls-concept:C0026809 | lld:lifeskim |
| pubmed-article:8933368 | lifeskim:mentions | umls-concept:C0006104 | lld:lifeskim |
| pubmed-article:8933368 | lifeskim:mentions | umls-concept:C0206116 | lld:lifeskim |
| pubmed-article:8933368 | lifeskim:mentions | umls-concept:C0178719 | lld:lifeskim |
| pubmed-article:8933368 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:8933368 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:8933368 | pubmed:dateCreated | 1997-3-11 | lld:pubmed |
| pubmed-article:8933368 | pubmed:abstractText | Intracellular pH (pHi) and the mechanisms of pHi regulation have been investigated in cultured microglial cells from mouse brain using the pH-sensitive fluorescent dye 2',7'-bis-(2-carboxyethyl)-5-(6)-carboxyfluorescein (BCECF). Cells were acidified by a pulse of NH4+ (4-5 min; 20 mM) and the subsequent pHi recovery from an acidification was studied. In HCO3(-)-free saline, pH regulation was dependent on extracellular [Na+] and sensitive to amiloride, indicating the involvement of the Na+/H+ exchanger. In HCO3(-)-containing solution 2 mM amiloride slowed but did not block pHi recovery; the recovery however was dependent on extracellular [Na+] and sensitive to 0.3 mM DIDS, suggesting the presence of Na+/HCO3 cotransporter and/or Na(+)-dependent Cl-/HCO3-exchanger. The involvement of a Na-dependent Cl-/HCO3-exchanger was inferred from the observation that removal of Cl- or application of 1 mM furosemide decreased but did not block the recovery rate. Increasing [K+]0 resulted in an alkalinization by a process that was neither HCO3- nor Na(+)-dependent, nor DIDS- and amiloride-inhibitable. In conclusion, microglial cells express a distinct set of pH regulatory carriers which control for a defined level of pHi. An increase in [K+]0 can offset this level. | lld:pubmed |
| pubmed-article:8933368 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8933368 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8933368 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8933368 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8933368 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8933368 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8933368 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8933368 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8933368 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8933368 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8933368 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8933368 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8933368 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8933368 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8933368 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8933368 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:8933368 | pubmed:issn | 0360-4012 | lld:pubmed |
| pubmed-article:8933368 | pubmed:author | pubmed-author:KettenmannHH | lld:pubmed |
| pubmed-article:8933368 | pubmed:author | pubmed-author:OhlemeyerCC | lld:pubmed |
| pubmed-article:8933368 | pubmed:author | pubmed-author:DahlN HNH | lld:pubmed |
| pubmed-article:8933368 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8933368 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:8933368 | pubmed:volume | 46 | lld:pubmed |
| pubmed-article:8933368 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8933368 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8933368 | pubmed:pagination | 294-304 | lld:pubmed |
| pubmed-article:8933368 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:8933368 | pubmed:meshHeading | pubmed-meshheading:8933368-... | lld:pubmed |
| pubmed-article:8933368 | pubmed:meshHeading | pubmed-meshheading:8933368-... | lld:pubmed |
| pubmed-article:8933368 | pubmed:meshHeading | pubmed-meshheading:8933368-... | lld:pubmed |
| pubmed-article:8933368 | pubmed:meshHeading | pubmed-meshheading:8933368-... | lld:pubmed |
| pubmed-article:8933368 | pubmed:meshHeading | pubmed-meshheading:8933368-... | lld:pubmed |
| pubmed-article:8933368 | pubmed:meshHeading | pubmed-meshheading:8933368-... | lld:pubmed |
| pubmed-article:8933368 | pubmed:meshHeading | pubmed-meshheading:8933368-... | lld:pubmed |
| pubmed-article:8933368 | pubmed:meshHeading | pubmed-meshheading:8933368-... | lld:pubmed |
| pubmed-article:8933368 | pubmed:meshHeading | pubmed-meshheading:8933368-... | lld:pubmed |
| pubmed-article:8933368 | pubmed:meshHeading | pubmed-meshheading:8933368-... | lld:pubmed |
| pubmed-article:8933368 | pubmed:meshHeading | pubmed-meshheading:8933368-... | lld:pubmed |
| pubmed-article:8933368 | pubmed:meshHeading | pubmed-meshheading:8933368-... | lld:pubmed |
| pubmed-article:8933368 | pubmed:meshHeading | pubmed-meshheading:8933368-... | lld:pubmed |
| pubmed-article:8933368 | pubmed:meshHeading | pubmed-meshheading:8933368-... | lld:pubmed |
| pubmed-article:8933368 | pubmed:meshHeading | pubmed-meshheading:8933368-... | lld:pubmed |
| pubmed-article:8933368 | pubmed:meshHeading | pubmed-meshheading:8933368-... | lld:pubmed |
| pubmed-article:8933368 | pubmed:meshHeading | pubmed-meshheading:8933368-... | lld:pubmed |
| pubmed-article:8933368 | pubmed:year | 1996 | lld:pubmed |
| pubmed-article:8933368 | pubmed:articleTitle | Intracellular pH regulation in cultured microglial cells from mouse brain. | lld:pubmed |
| pubmed-article:8933368 | pubmed:affiliation | Max Delbrück Center for Moleculare Medicine, Cellular Neuroscience, Berlin-Buch, Germany. | lld:pubmed |
| pubmed-article:8933368 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8933368 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8933368 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8933368 | lld:pubmed |
