Linked Life Data

8930747

Source:http://linkedlifedata.com/resource/pubmed/id/8930747

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1997-2-18
pubmed:abstractText
The recent increased use of cell cultures to model physiological events, in particular signal transduction and traumatic injury, has produced a need for a quantitative, high-performance liquid chromatographic separation of neutral lipid classes with a high degree of resolution and reproducibility. We report an isocratic separation using a Phenomenex Selectosil silica column (5 microns). Two solvents were used, 1.2% 2-propanol in n-hexane containing 0.1% acetic acid (90%) and n-hexane (10%) at a flow-rate of 0.6 ml/min. Column temperature was maintained at 55 degrees C and this temperature was critical for baseline resolution of 1,3-diacylglycerol and cholesterol. The use of 10% n-hexane permitted the resolution of low polarity compounds such as butylated hydroxytoluene, triacylglycerols and cholesteryl esters. All of the detectors used produced standard curves with linearity over a wide concentration range.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1572-6495
pubmed:author
pubmed:issnType
Print
pubmed:day
11
pubmed:volume
685
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
9-14
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Separation of neutral lipids by high-performance liquid chromatography: quantification by ultraviolet, light scattering and fluorescence detection.
pubmed:affiliation
Department of Medical Biochemistry, Ohio State University, Columbus 43210, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.