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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1996-11-27
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pubmed:abstractText |
A conjugational gene transfer system consisting of donor and recipient strains has been developed for genetic analysis of Vibrio cholerae 0139 serogroup, a new cholera agent. Donor strains constructed using the Tn5-Mob carrying the origin of transfer (ori T) of plasmid RP4 and helper plasmid pRP4-4 were able to perform a directed transfer of chromosomal markers. Recipient strains carried mutations in auxotrophic genes as well as in virulence genes. Based on this gene transfer system, a genetic map of V. cholerae chromosome has been created showing the order of 17 gene markers. Relationship between the genetic structure of V. cholerae 0139 and 01 is discussed.
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pubmed:language |
rus
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
0208-0613
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
14-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8927057-Chromosome Mapping,
pubmed-meshheading:8927057-Chromosomes, Bacterial,
pubmed-meshheading:8927057-Cloning, Molecular,
pubmed-meshheading:8927057-Conjugation, Genetic,
pubmed-meshheading:8927057-DNA Transposable Elements,
pubmed-meshheading:8927057-Gene Transfer Techniques,
pubmed-meshheading:8927057-Plasmids,
pubmed-meshheading:8927057-Vibrio cholerae
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pubmed:articleTitle |
[Creating a system of conjugated chromosome transfer and genetic mapping of Vibrio cholerae serogroup O139 chromosomes].
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pubmed:publicationType |
Journal Article,
English Abstract
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