Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
10
|
| pubmed:dateCreated |
1997-2-10
|
| pubmed:abstractText |
The CD28/CTLA-4 ligands, B7-1 (CD80) and B7-2 (CD86), provide a co-stimulatory signal necessary for optimal T cell activation. We have examined the effect of blocking B7-1 and B7-2 in an in vitro system using ovalbumin-specific T cells from alpha beta TCR-transgenic mice. This system allowed us to examine the interaction of B7 co-stimulators on physiologic antigen-presenting cells (APC) with antigen-specific T helper precursor (ThP) cells. We report that blocking Thp/B7-1 or B7-2 interactions in a primary response differentially affects the cytokine profile observed in a secondary stimulation, even in the absence of additional anti-B7 antibody. Engagement of B7-2 in the primary stimulation was found to be essential for production of the Th2 cytokine, IL-4, but not the Th1 cytokines, IL-2 and IFN-gamma, in a secondary stimulation. Conversely, inclusion of the anti-B7-1 mAb in cultures using highly purified naive T cells increased levels of IL-4 and significantly depressed levels of IFN-gamma, upon re-stimulation. The effect of the anti-B7-2 mAb in reducing IL-4 production could be overcome by the addition of recombinant IL-4 in the primary stimulation. The effects of the anti-B7-2 mAb appear to be due to blocking and not cross-linking, as F(ab) fragments mimicked the intact antibody. Taken together, our data demonstrate that the interaction between Thp and B7-2 favors the development of Th2 cells.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Blocking,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD80,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD86,
http://linkedlifedata.com/resource/pubmed/chemical/Cd86 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Fab Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0953-8178
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
8
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1549-60
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8921434-Animals,
pubmed-meshheading:8921434-Antibodies, Blocking,
pubmed-meshheading:8921434-Antigens, CD,
pubmed-meshheading:8921434-Antigens, CD80,
pubmed-meshheading:8921434-Antigens, CD86,
pubmed-meshheading:8921434-Cell Differentiation,
pubmed-meshheading:8921434-Cell Separation,
pubmed-meshheading:8921434-Immunoglobulin Fab Fragments,
pubmed-meshheading:8921434-Interleukin-4,
pubmed-meshheading:8921434-Lymphocyte Activation,
pubmed-meshheading:8921434-Membrane Glycoproteins,
pubmed-meshheading:8921434-Mice,
pubmed-meshheading:8921434-Mice, Inbred BALB C,
pubmed-meshheading:8921434-Mice, Transgenic,
pubmed-meshheading:8921434-Recombinant Proteins,
pubmed-meshheading:8921434-Spleen,
pubmed-meshheading:8921434-T-Lymphocytes,
pubmed-meshheading:8921434-T-Lymphocytes, Helper-Inducer
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
B7-2 (CD86) is essential for the development of IL-4-producing T cells.
|
| pubmed:affiliation |
Department of Cancer Biology, Harvard School of Public Health, Boston, MA 02115, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|