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| pubmed-article:8912697 | lifeskim:mentions | umls-concept:C1006981 | lld:lifeskim |
| pubmed-article:8912697 | lifeskim:mentions | umls-concept:C0009015 | lld:lifeskim |
| pubmed-article:8912697 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:8912697 | lifeskim:mentions | umls-concept:C1514559 | lld:lifeskim |
| pubmed-article:8912697 | lifeskim:mentions | umls-concept:C0456387 | lld:lifeskim |
| pubmed-article:8912697 | lifeskim:mentions | umls-concept:C1561491 | lld:lifeskim |
| pubmed-article:8912697 | lifeskim:mentions | umls-concept:C0051154 | lld:lifeskim |
| pubmed-article:8912697 | pubmed:dateCreated | 1996-12-26 | lld:pubmed |
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| pubmed-article:8912697 | pubmed:abstractText | A gene of Pseudomonas alginovora, called aly, has been cloned in Escherichia coli using a battery of PCR techniques and sequenced. It encodes a 210-amino-acid alginate lyase (EC 4.2.2.3), Aly, in the form of a 233-amino-acid precursor. P. alginovora Aly has been overproduced in E. coli with a His-tag sequence fused at the C-terminal end under conditions in which the formation of inclusion bodies is avoided. His-tagged P. alginovora Aly has the same enzymic properties as the wild-type enzyme and has the specificity of a mannuronate lyase. It can be purified in a one-step procedure by affinity chromatography on Ni(2+)-nitriloacetate resin. The yield is of 5 mg of enzyme per litre of culture. The amplification factor is 12.5 compared with the level of production by wild-type P. alginovora. The six alginate lyases of known primary structure fall into three distinct classes, one of which comprises the pair P. alginovora Aly and Klebsiella pneumoniae Aly. | lld:pubmed |
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| pubmed-article:8912697 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8912697 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8912697 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:8912697 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8912697 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8912697 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:8912697 | pubmed:issn | 0264-6021 | lld:pubmed |
| pubmed-article:8912697 | pubmed:author | pubmed-author:GuinandMM | lld:pubmed |
| pubmed-article:8912697 | pubmed:author | pubmed-author:GhuysenJ MJM | lld:pubmed |
| pubmed-article:8912697 | pubmed:author | pubmed-author:DuezCC | lld:pubmed |
| pubmed-article:8912697 | pubmed:author | pubmed-author:WallaceOO | lld:pubmed |
| pubmed-article:8912697 | pubmed:author | pubmed-author:HenrissatBB | lld:pubmed |
| pubmed-article:8912697 | pubmed:author | pubmed-author:BarbeyronTT | lld:pubmed |
| pubmed-article:8912697 | pubmed:author | pubmed-author:PotinPP | lld:pubmed |
| pubmed-article:8912697 | pubmed:author | pubmed-author:ChavagnatFF | lld:pubmed |
| pubmed-article:8912697 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8912697 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:8912697 | pubmed:volume | 319 ( Pt 2) | lld:pubmed |
| pubmed-article:8912697 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8912697 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8912697 | pubmed:pagination | 575-83 | lld:pubmed |
| pubmed-article:8912697 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:8912697 | pubmed:meshHeading | pubmed-meshheading:8912697-... | lld:pubmed |
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| pubmed-article:8912697 | pubmed:meshHeading | pubmed-meshheading:8912697-... | lld:pubmed |
| pubmed-article:8912697 | pubmed:meshHeading | pubmed-meshheading:8912697-... | lld:pubmed |
| pubmed-article:8912697 | pubmed:meshHeading | pubmed-meshheading:8912697-... | lld:pubmed |
| pubmed-article:8912697 | pubmed:meshHeading | pubmed-meshheading:8912697-... | lld:pubmed |
| pubmed-article:8912697 | pubmed:meshHeading | pubmed-meshheading:8912697-... | lld:pubmed |
| pubmed-article:8912697 | pubmed:meshHeading | pubmed-meshheading:8912697-... | lld:pubmed |
| pubmed-article:8912697 | pubmed:meshHeading | pubmed-meshheading:8912697-... | lld:pubmed |
| pubmed-article:8912697 | pubmed:meshHeading | pubmed-meshheading:8912697-... | lld:pubmed |
| pubmed-article:8912697 | pubmed:year | 1996 | lld:pubmed |
| pubmed-article:8912697 | pubmed:articleTitle | Cloning, sequencing and overexpression in Escherichia coli of the alginatelyase-encoding aly gene of Pseudomonas alginovora: identification of three classes of alginate lyases. | lld:pubmed |
| pubmed-article:8912697 | pubmed:affiliation | Laboratoire de Biochimie Analytique et Synthèse Bioorganique, Université Claude Bernard, Villeurbanne, France. | lld:pubmed |
| pubmed-article:8912697 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8912697 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8912697 | lld:pubmed |
