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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1996-12-26
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pubmed:databankReference |
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pubmed:abstractText |
A gene of Pseudomonas alginovora, called aly, has been cloned in Escherichia coli using a battery of PCR techniques and sequenced. It encodes a 210-amino-acid alginate lyase (EC 4.2.2.3), Aly, in the form of a 233-amino-acid precursor. P. alginovora Aly has been overproduced in E. coli with a His-tag sequence fused at the C-terminal end under conditions in which the formation of inclusion bodies is avoided. His-tagged P. alginovora Aly has the same enzymic properties as the wild-type enzyme and has the specificity of a mannuronate lyase. It can be purified in a one-step procedure by affinity chromatography on Ni(2+)-nitriloacetate resin. The yield is of 5 mg of enzyme per litre of culture. The amplification factor is 12.5 compared with the level of production by wild-type P. alginovora. The six alginate lyases of known primary structure fall into three distinct classes, one of which comprises the pair P. alginovora Aly and Klebsiella pneumoniae Aly.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8912697-1516726,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8912697-1526969,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8912697-1886523,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8912697-2126461,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8912697-2170123,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8912697-2516117,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8912697-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8912697-3098421,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8912697-3141564,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8912697-3155514,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8912697-3162770,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8912697-3344219,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8912697-3550697,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8912697-3611052,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8912697-3678489,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8912697-388356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8912697-4204102,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8912697-646115,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8912697-6546423,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8912697-7356324,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8912697-7634076,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8912697-7654407,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8912697-7664116,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8912697-7705601,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8912697-7716248,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8912697-8081738,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8912697-8200539,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8912697-8319887,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8912697-8335634,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8912697-8336113,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8912697-8370530,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8912697-942051,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8912697-94251
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
319 ( Pt 2)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
575-83
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8912697-Amino Acid Sequence,
pubmed-meshheading:8912697-Base Sequence,
pubmed-meshheading:8912697-Cloning, Molecular,
pubmed-meshheading:8912697-Escherichia coli,
pubmed-meshheading:8912697-Gene Transfer Techniques,
pubmed-meshheading:8912697-Molecular Sequence Data,
pubmed-meshheading:8912697-Polysaccharide-Lyases,
pubmed-meshheading:8912697-Pseudomonas,
pubmed-meshheading:8912697-Sequence Alignment,
pubmed-meshheading:8912697-Sequence Analysis
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pubmed:year |
1996
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pubmed:articleTitle |
Cloning, sequencing and overexpression in Escherichia coli of the alginatelyase-encoding aly gene of Pseudomonas alginovora: identification of three classes of alginate lyases.
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pubmed:affiliation |
Laboratoire de Biochimie Analytique et Synthèse Bioorganique, Université Claude Bernard, Villeurbanne, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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