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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1996-12-13
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pubmed:abstractText |
Fibroblasts cultivated in a collagen matrix exhibit a large decrease in the synthesis of most proteins, depending on transcriptional and posttranscriptional controls. We have previously shown that ribosomal RNA content and half-life were decreased in collagen lattice cultures. Here, we cultivated human dermal fibroblasts in monolayers and in lattices and studied by competitive RT-PCR analysis the expression of the nucleolar proteins nucleolin and fibrillarin, two key factors in ribosome processing and association. Nucleolin expression was found increased, and fibrillarin expression decreased, in collagen-lattice vs monolayer-cultured fibroblasts, with some variability according to the strains (+25 to +250% and -40 to -60%, respectively). These data suggest that a possible trouble of the association between neosynthesized rRNA and nucleolar proteins is, at least partly, responsible for the inhibition of protein synthesis induced by the extracellular matrix.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Chromosomal Proteins, Non-Histone,
http://linkedlifedata.com/resource/pubmed/chemical/Collagen,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA Precursors,
http://linkedlifedata.com/resource/pubmed/chemical/RNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/fibrillarin,
http://linkedlifedata.com/resource/pubmed/chemical/nucleolin
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0006-291X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
228
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
94-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8912641-Cells, Cultured,
pubmed-meshheading:8912641-Chromosomal Proteins, Non-Histone,
pubmed-meshheading:8912641-Collagen,
pubmed-meshheading:8912641-Extracellular Matrix,
pubmed-meshheading:8912641-Fibroblasts,
pubmed-meshheading:8912641-Humans,
pubmed-meshheading:8912641-Nuclear Proteins,
pubmed-meshheading:8912641-Phosphoproteins,
pubmed-meshheading:8912641-Polymerase Chain Reaction,
pubmed-meshheading:8912641-RNA, Messenger,
pubmed-meshheading:8912641-RNA Precursors,
pubmed-meshheading:8912641-RNA-Binding Proteins
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pubmed:year |
1996
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pubmed:articleTitle |
Modulation of protein synthesis by extracellular matrix: potential involvement of two nucleolar proteins, nucleolin and fibrillarin.
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pubmed:affiliation |
Laboratory of Biochemistry and Molecular Biology, CNRS EP 89, IFR 53-Biomolecules, Faculty of Medicine, University of Reims Champagne-Ardenne, France.
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pubmed:publicationType |
Journal Article,
Comparative Study
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