Linked Life Data

8892611

Source:http://linkedlifedata.com/resource/pubmed/id/8892611

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1996-12-13
pubmed:abstractText
Previous work indicated that a subclass of mouse spleen dendritic cells (DC), those bearing CD8alpha, expresses the Fas ligand and restricts peripheral CD4 T cell responses by initiating Fas-mediated apoptosis. To determine whether a similar regulation applies to CD8 T cells, they were purified from normal or from TCR-transgenic mice, and then cultured with purified splenic CD8+ DC or CD8- DC presenting either alloantigens or the specific Ag for the TCR transgene. In all systems studied, the proliferative response of CD8 T cells was markedly less on stimulation with CD8+ DC compared with conventional CD8- DC. However, the basis of this restricted proliferation in response to CD8+ DC was totally different for CD8 T cells than for CD4 T cells. The reduced proliferation of CD8 T cells occurred later in the response than with CD4 T cells. In contrast with CD4 T cells, the reduced proliferation of CD8 T cells occurred even with T cells from Fas-deficient Ipr mice, or with DC from Fas ligand-deficient gld mice, indicating that Fas-induced apoptosis was not involved. Also, in contrast with CD4 T cells, the reduced proliferation of CD8 T cells was completely reversed by the addition of exogenous IL-2. Furthermore, cultures of CD8 T cells with CD8+ DC were found to be deficient in IL-2 production. Accordingly, although CD8+ DC are very efficient at stimulating CD8 T cells into cell division, they are deficient at stimulating endogenous cytokine production. The implications of these different DC regulatory systems are discussed.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
157
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3819-27
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:8892611-Animals, pubmed-meshheading:8892611-Antigen Presentation, pubmed-meshheading:8892611-Antigens, CD95, pubmed-meshheading:8892611-Apoptosis, pubmed-meshheading:8892611-CD4-Positive T-Lymphocytes, pubmed-meshheading:8892611-CD8-Positive T-Lymphocytes, pubmed-meshheading:8892611-Cell Cycle, pubmed-meshheading:8892611-Dendritic Cells, pubmed-meshheading:8892611-Fas Ligand Protein, pubmed-meshheading:8892611-Gene Expression Regulation, pubmed-meshheading:8892611-Interleukin-2, pubmed-meshheading:8892611-Lymphocyte Activation, pubmed-meshheading:8892611-Lymphocyte Culture Test, Mixed, pubmed-meshheading:8892611-Membrane Glycoproteins, pubmed-meshheading:8892611-Mice, pubmed-meshheading:8892611-Mice, Inbred Strains, pubmed-meshheading:8892611-Mice, Mutant Strains, pubmed-meshheading:8892611-Mice, Transgenic, pubmed-meshheading:8892611-Specific Pathogen-Free Organisms, pubmed-meshheading:8892611-Spleen
pubmed:year
1996
pubmed:articleTitle
A subclass of dendritic cells regulates the response of naive CD8 T cells by limiting their IL-2 production.
pubmed:affiliation
The Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria, Australia.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't