Linked Life Data

8882521

Source:http://linkedlifedata.com/resource/pubmed/id/8882521

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1996-12-11
pubmed:abstractText
A human autoimmune serum is used to characterize a protein which is located within a dot-like structure of the interphase nucleus. The dots are located in the nucleoplasma outside the nucleoli. The dot-like structure could be observed on a variety of human substrates (HEp-2, HepG2, HeLa, Molt-4, WI-38 cell lines, peripheral blood lymphocytes), mouse cell lines and tissues (3T3, L929, spleen sections), rat sections, marsupial PtK2 cells and hamster cell lines. The range of the number of dots and their size differed between cells and cell lines and varied between 1 and 24. The antigen could be identified as a 53 kDa protein with a pI of 8.7 and was named NDP53. Digestion experiments suggested that the protein is not associated with DNA or RNA, but is associated with the nuclear matrix. Immunelectron microscopy using ultra-thin sections revealed filamentous structures with a diameter of 0.1 to 0.6 microns. The antigen colocalizes with Sp100, PML and NDP55, which are part of a multiprotein complex known as PML oncogenic domain (POD), nuclear bodies, Kr bodies or ND10.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0248-4900
pubmed:author
pubmed:issnType
Print
pubmed:volume
85
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
77-86
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
A human autoantibody recognizing nuclear matrix-associated nuclear protein localized in dot structures.
pubmed:affiliation
Internal Medicine I, University of Saarland, Homburg-Saar, Germany.
pubmed:publicationType
Journal Article