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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
21
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pubmed:dateCreated |
1996-12-4
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pubmed:abstractText |
Damage to actively transcribed DNA is preferentially repaired by the transcription-coupled repair (TCR) system. TCR requires RNA polymerase II (Pol II), but the mechanism by which repair enzymes preferentially recognize and repair DNA lesions on Pol II-transcribed genes is incompletely understood. Herein we demonstrate that a fraction of the large subunit of Pol II (Pol II LS) is ubiquitinated after exposing cells to UV-radiation or cisplatin but not several other DNA damaging agents. This novel covalent modification of Pol II LS occurs within 15 min of exposing cells to UV-radiation and persists for about 8-12 hr. Ubiquitinated Pol II LS is also phosphorylated on the C-terminal domain. UV-induced ubiquitination of Pol II LS is deficient in fibroblasts from individuals with two forms of Cockayne syndrome (CS-A and CS-B), a rare disorder in which TCR is disrupted. UV-induced ubiquitination of Pol II LS can be restored by introducing cDNA constructs encoding the CSA or CSB genes, respectively, into CS-A or CS-B fibroblasts. These results suggest that ubiquitination of Pol II LS plays a role in the recognition and/or repair of damage to actively transcribed genes. Alternatively, these findings may reflect a role played by the CSA and CSB gene products in transcription.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876179-1312335,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876179-1336336,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876179-1339317,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876179-1429664,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876179-1438266,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876179-1649389,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876179-1717990,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876179-1906131,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876179-2822254,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876179-3020426,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876179-3149714,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876179-3306404,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876179-3459159,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876179-3664636,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876179-7536746,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876179-7587084,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876179-7638163,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876179-7662365,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876179-7664335,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876179-7711060,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876179-7770913,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876179-7801121,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876179-7862120,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876179-7926769,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876179-7980491,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876179-8078911,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876179-8087846,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876179-8168482,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876179-8202161,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876179-8533150,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876179-8533154,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876179-8533159,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876179-8550554,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876179-8601303,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876179-8601309,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876179-8882582
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
93
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
11586-90
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8876179-Cell Line,
pubmed-meshheading:8876179-Cisplatin,
pubmed-meshheading:8876179-Cockayne Syndrome,
pubmed-meshheading:8876179-DNA Damage,
pubmed-meshheading:8876179-DNA Repair,
pubmed-meshheading:8876179-HeLa Cells,
pubmed-meshheading:8876179-Humans,
pubmed-meshheading:8876179-Hydrogen Peroxide,
pubmed-meshheading:8876179-Phosphorylation,
pubmed-meshheading:8876179-RNA Polymerase II,
pubmed-meshheading:8876179-Recombinant Proteins,
pubmed-meshheading:8876179-Transcription, Genetic,
pubmed-meshheading:8876179-Transfection,
pubmed-meshheading:8876179-Ubiquitins,
pubmed-meshheading:8876179-Ultraviolet Rays
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pubmed:year |
1996
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pubmed:articleTitle |
UV-induced ubiquitination of RNA polymerase II: a novel modification deficient in Cockayne syndrome cells.
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pubmed:affiliation |
Department of Pathology, Yale University School of Medicine, New Haven, CT 06520, USA. bregman@aecom.yu.edu
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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