Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1997-1-23
pubmed:abstractText
Mutations in the gyrA gene resulting in amino acid changes at Ser-91 and Asp-95 are significantly associated with decreased susceptibilities to quinolones in Neisseria gonorrhoeae. To detect these mutations, we developed a rapid and simple assay based on amplification of the region of the gyrA gene containing the mutation sites by PCR and digestion of the PCR product with a restriction enzyme. A naturally occurring HinfI restriction site was present in the region containing the Ser-91 codon, and an artificial HinfI restriction site was created in the region containing the Asp-95 codon by the method of primer-specified restriction site modification. The mutations generating alterations at Ser-91 and Asp-95 were detected as restriction fragment length polymorphisms of the PCR products digested with HinfI. Fifty-five clinical strains of N. gonorrhoeae were examined for mutations in the gyrA gene by this method. Mutations at Ser-91 and/or Asp-95 were detected in all the 31 strains in which the mutations had been confirmed by DNA sequencing. Our method allows simultaneous testing of a large number of strains and provides results within 8 h. This rapid and simple assay could be a useful screening device for genetic alterations associated with decreased susceptibilities to quinolones in N. gonorrhoeae and could facilitate epidemiological studies on clinical isolates of N. gonorrhoeae with decreased susceptibilities to quinolones.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8862594-1662027, http://linkedlifedata.com/resource/pubmed/commentcorrection/8862594-1736071, http://linkedlifedata.com/resource/pubmed/commentcorrection/8862594-1761455, http://linkedlifedata.com/resource/pubmed/commentcorrection/8862594-1842918, http://linkedlifedata.com/resource/pubmed/commentcorrection/8862594-1906260, http://linkedlifedata.com/resource/pubmed/commentcorrection/8862594-1967354, http://linkedlifedata.com/resource/pubmed/commentcorrection/8862594-1967452, http://linkedlifedata.com/resource/pubmed/commentcorrection/8862594-1987461, http://linkedlifedata.com/resource/pubmed/commentcorrection/8862594-2168148, http://linkedlifedata.com/resource/pubmed/commentcorrection/8862594-2174869, http://linkedlifedata.com/resource/pubmed/commentcorrection/8862594-2687159, http://linkedlifedata.com/resource/pubmed/commentcorrection/8862594-2726503, http://linkedlifedata.com/resource/pubmed/commentcorrection/8862594-2828631, http://linkedlifedata.com/resource/pubmed/commentcorrection/8862594-7502185, http://linkedlifedata.com/resource/pubmed/commentcorrection/8862594-7726535, http://linkedlifedata.com/resource/pubmed/commentcorrection/8862594-7830580, http://linkedlifedata.com/resource/pubmed/commentcorrection/8862594-7871449, http://linkedlifedata.com/resource/pubmed/commentcorrection/8862594-7959709, http://linkedlifedata.com/resource/pubmed/commentcorrection/8862594-7959720, http://linkedlifedata.com/resource/pubmed/commentcorrection/8862594-8031045, http://linkedlifedata.com/resource/pubmed/commentcorrection/8862594-8206482, http://linkedlifedata.com/resource/pubmed/commentcorrection/8862594-8384814, http://linkedlifedata.com/resource/pubmed/commentcorrection/8862594-8540700, http://linkedlifedata.com/resource/pubmed/commentcorrection/8862594-8849219
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
34
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2255-8
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Rapid detection of point mutations of the Neisseria gonorrhoeae gyrA gene associated with decreased susceptibilities to quinolones.
pubmed:affiliation
Department of Urology, Gifu University School of Medicine, Japan.
pubmed:publicationType
Journal Article