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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1996-12-16
|
| pubmed:databankReference | |
| pubmed:abstractText |
The origin recognition complex (ORC) binds to origins of replication in budding yeast. We have cloned a Xenopus homolog of the largest ORC polypeptide (XORC1). Immunodepletion of XOrc1 from Xenopus egg extracts blocks the initiation of DNA replication. We have purified Xenopus ORC, consisting of a protein complex similar to yeast ORC. In Xenopus egg extracts, ORC associates with chromatin throughout G1 and S phases. RLF-M, a component of the replication licensing system, also associates with chromatin early in the cell cycle but dissociates during S phase. We show that the assembly of RLF-M onto chromatin is dependent on the presence of chromatin-bound ORC, leading to sequential assembly of initiation proteins onto replication origins during the cell cycle.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0092-8674
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
18
|
| pubmed:volume |
87
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
287-96
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8861912-Amino Acid Sequence,
pubmed-meshheading:8861912-Animals,
pubmed-meshheading:8861912-Base Sequence,
pubmed-meshheading:8861912-Cell Cycle,
pubmed-meshheading:8861912-Cell-Free System,
pubmed-meshheading:8861912-Chromatin,
pubmed-meshheading:8861912-Cloning, Molecular,
pubmed-meshheading:8861912-DNA Replication,
pubmed-meshheading:8861912-DNA-Binding Proteins,
pubmed-meshheading:8861912-Humans,
pubmed-meshheading:8861912-Immunologic Techniques,
pubmed-meshheading:8861912-Male,
pubmed-meshheading:8861912-Molecular Sequence Data,
pubmed-meshheading:8861912-Oocytes,
pubmed-meshheading:8861912-Origin Recognition Complex,
pubmed-meshheading:8861912-Sequence Alignment,
pubmed-meshheading:8861912-Spermatozoa,
pubmed-meshheading:8861912-Xenopus Proteins,
pubmed-meshheading:8861912-Xenopus laevis
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Interaction between the origin recognition complex and the replication licensing system in Xenopus.
|
| pubmed:affiliation |
Imperial Cancer Research Fund, Clare Hall Laboratories, Herts, United Kingdom.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|