|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
1996-11-25
|
|
pubmed:abstractText |
We have monitored kinetics of fusion between cell pairs consisting of a single influenza hemaglutinin (HA)-expressing cell and a single erythrocyte (RBC) that had been labeled with both a fluorescent lipid (Dil) in the membrane and a fluorescent solute (calcein) in the aqueous space. Initial fusion pore opening between the RBC and HA-expressing cell produced a change in RBC membrane potential (delta psi) that was monitored by a decrease in Dil fluorescence. This event was followed by two distinct stages of fusion pore dilation: the flux of fluorescent lipid (phi L) and the flux of a large aqueous fluorescent dye (phi s). We have analyzed the kinetics of events that occur as a result of transitions between a fusion pore (FP) and a solute permissive fusion pore (FPs). Our data are consistent with a fusion pore comprising six HA trimers.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8858163-12991237,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8858163-1397318,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8858163-1420930,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8858163-1469040,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8858163-1741588,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8858163-1850019,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8858163-2023911,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8858163-2055263,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8858163-2271610,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8858163-2308945,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8858163-2331370,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8858163-2442614,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8858163-2453272,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8858163-2586627,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8858163-2611351,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8858163-2745545,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8858163-2917985,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8858163-3307918,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8858163-3470745,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8858163-4851321,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8858163-574515,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8858163-6265470,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8858163-6504157,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8858163-7464906,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8858163-7593189,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8858163-7632704,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8858163-7690263,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8858163-7767373,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8858163-7767376,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8858163-7791897,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8858163-7806567,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8858163-7843286,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8858163-7909233,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8858163-8028665,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8858163-8113761,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8858163-8133242,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8858163-8133243,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8858163-8201976,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8858163-8293471,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8858163-8298039,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8858163-8361385,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8858163-8387488,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8858163-8486735,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8858163-8510561,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8858163-8636231
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Oct
|
|
pubmed:issn |
0021-9525
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
135
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
63-71
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:8858163-3T3 Cells,
pubmed-meshheading:8858163-Animals,
pubmed-meshheading:8858163-CHO Cells,
pubmed-meshheading:8858163-Cell Fusion,
pubmed-meshheading:8858163-Cricetinae,
pubmed-meshheading:8858163-Erythrocyte Membrane,
pubmed-meshheading:8858163-Fluoresceins,
pubmed-meshheading:8858163-Fluorescent Dyes,
pubmed-meshheading:8858163-Hemagglutinin Glycoproteins, Influenza Virus,
pubmed-meshheading:8858163-Humans,
pubmed-meshheading:8858163-Kinetics,
pubmed-meshheading:8858163-Membrane Fusion,
pubmed-meshheading:8858163-Membrane Potentials,
pubmed-meshheading:8858163-Mice,
pubmed-meshheading:8858163-Microscopy, Video,
pubmed-meshheading:8858163-Temperature
|
|
pubmed:year |
1996
|
|
pubmed:articleTitle |
Dilation of the influenza hemagglutinin fusion pore revealed by the kinetics of individual cell-cell fusion events.
|
|
pubmed:affiliation |
Section on Membrane Structure and Function, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA. blumen@helix.nih.gov
|
|
pubmed:publicationType |
Journal Article
|
