|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
1997-1-10
|
|
pubmed:abstractText |
We have investigated endothelial function in a two-hit model of multiple organ failure. Male Fischer rats were subjected to 20 min of partial hepatic ischemia followed by reperfusion and administration of .5 mg/kg Salmonella enteritidis endotoxin at 30 min of reperfusion. After either 4 or 24 h of reperfusion, rings of aorta were prepared and suspended in bioassay baths, contracted with phenylephrine, and examined for endothelium-dependent relaxation in response to acetylcholine and endothelium-independent relaxation using nitroglycerin. Endothelium-dependent relaxation to acetylcholine was impaired in rings from animals exposed to endotoxin-enhanced reperfusion injury at both 4 h and 24 h. At 24 h of reperfusion the EC50 for acetylcholine relaxation was significantly increased from 45 +/- 8 nM to 258 +/- 105 nM. Endothelium-independent relaxation to nitroglycerin was not affected. The 21-aminosteroid Tirilazad mesylate (U-74006F) prevented endothelial dysfunction; at 24 h of reperfusion the EC50 for acetylcholine relaxation in U-74006F-treated animals was 55 +/- 8 nM. Thus, endothelial function is impaired in this model of multiple organ failure and this impairment is prevented by Tirilazad mesylate.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Aug
|
|
pubmed:issn |
1073-2322
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
6
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
106-11
|
|
pubmed:dateRevised |
2003-11-14
|
|
pubmed:meshHeading |
pubmed-meshheading:8856844-Acetylcholine,
pubmed-meshheading:8856844-Animals,
pubmed-meshheading:8856844-Antioxidants,
pubmed-meshheading:8856844-Aorta, Thoracic,
pubmed-meshheading:8856844-Bacterial Toxins,
pubmed-meshheading:8856844-Constriction,
pubmed-meshheading:8856844-Disease Models, Animal,
pubmed-meshheading:8856844-Endothelium, Vascular,
pubmed-meshheading:8856844-Endotoxins,
pubmed-meshheading:8856844-Ischemia,
pubmed-meshheading:8856844-Lipid Peroxidation,
pubmed-meshheading:8856844-Liver,
pubmed-meshheading:8856844-Male,
pubmed-meshheading:8856844-Multiple Organ Failure,
pubmed-meshheading:8856844-Muscle, Smooth, Vascular,
pubmed-meshheading:8856844-Nitroglycerin,
pubmed-meshheading:8856844-Phenylephrine,
pubmed-meshheading:8856844-Pregnatrienes,
pubmed-meshheading:8856844-Rats,
pubmed-meshheading:8856844-Rats, Inbred F344,
pubmed-meshheading:8856844-Reperfusion Injury,
pubmed-meshheading:8856844-Vasodilation
|
|
pubmed:year |
1996
|
|
pubmed:articleTitle |
Effect of endotoxin-enhanced hepatic reperfusion injury on endothelium-dependent relaxation in rat aorta.
|
|
pubmed:affiliation |
Upjohn Company, Kalamazoo, Michigan 49001, USA.
|
|
pubmed:publicationType |
Journal Article
|
