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pubmed-article:8847525pubmed:abstractTextWe constructed a hybrid Moloney murine leukaemia virus (MoMLV) bearing both a chimeric CD4 and the wild-type MoMLV envelope protein (Env) on its surface. The chimeric molecule, consisting of a surface domain of CD4 and the C-terminal two-thirds of MLV Env, was expressed on the cell surface. When expressed in MoMLV-infected cells, the CD4-Env chimera was incorporated into the virion as efficiently as the wild-type MoMLV Env. The hybrid MoMLV could infect human HeLa cells (although not with high efficiency) only if the cells were expressing human immunodeficiency virus type 1 genome. This method of ligand incorporation into a virion may lead to a development of a cell-specific retroviral vector for targeting gene therapy.lld:pubmed
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pubmed-article:8847525pubmed:articleTitleTargeted infection of a retrovirus bearing a CD4-Env chimera into human cells expressing human immunodeficiency virus type 1.lld:pubmed
pubmed-article:8847525pubmed:affiliationDepartment of Bacteriology, Faculty of Medicine, University of Tokyo, Japan.lld:pubmed
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pubmed-article:8847525pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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